Overview
- External ontology
- TOGO:M285 — Modified MRS Medium
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- JCM Medium 291 lists 55.0 g Lactobacilli MRS broth from BD-Difco, 0.5 g L-Cysteine HCl x H2O, and 1.0 L Distilled water; the pH is adjusted to 6.5. Lactobacilli MRS broth is retained as a commercial medium product.
Composition
flowchart LR
CultureMech_009400["modified_mrs_medium"]
ing0_Lactobacilli_MRS_broth__BD_Difco_["Lactobacilli MRS broth (BD-Difco)"]
CultureMech_009400 --> ing0_Lactobacilli_MRS_broth__BD_Difco_
ing1_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_009400 --> ing1_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing1_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing2_Distilled_water["Distilled water"]
CultureMech_009400 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Lactobacilli MRS broth (BD-Difco) | 55.0 G_PER_L | JCM Medium 291 lists 55.0 g/L Lactobacilli MRS broth from BD-Difco; this commercial medium product is retained as an opaque component. | |
| 2 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
JCM Medium 291 lists 0.5 g/L L-Cysteine HCl x H2O. |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
JCM Medium 291 lists 1.0 L Distilled water. |
Preparation
- Suspend 55.0 g Lactobacilli MRS broth and 0.5 g L-Cysteine HCl x H2O in 1.0 L distilled water.
- Adjust pH to 6.5.
- Autoclave at 121 C for 15 min unless otherwise stated.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.765755Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M285 |
| 2026-01-28T19:48:13.984604Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.039422Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009400 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.978276+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified MRS Medium" to "modified_mrs_medium" for consistent matching |
| 2026-06-05T07:40:39.458782+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_jcm_j291_modified_mrs_score15.py | RESOLVED_TOGO_M285_SCORE15 | JCM Medium 291 lists 55.0 g Lactobacilli MRS broth from BD-Difco, 0.5 g L-Cysteine HCl x H2O, and 1.0 L Distilled water; the pH is adjusted to 6.5. Lactobacilli MRS broth is retained as a commercial medium product. Corrected the imported TOGO distilled-water unit to the 1.0 L JCM value, normalized L-Cysteine HCl x H2O, added JCM autoclave evidence, and linked the MediaDive J291 source duplicate. |