Overview
- External ontology
- TOGO:M286 — Lactobacillus Pontis Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M286 Original source: JCM - JCM_M292 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=292
Composition
flowchart LR
CultureMech_009403["lactobacillus_pontis_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009403 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Lactobacilli_MRS_broth__BD_Difco_["Lactobacilli MRS broth (BD-Difco)"]
CultureMech_009403 --> ing1_Lactobacilli_MRS_broth__BD_Difco_
ing2_Maltose["Maltose"]
CultureMech_009403 --> ing2_Maltose
CHEBI_18167["CHEBI:18167"]
ing2_Maltose -.-> CHEBI_18167
ing3_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009403 --> ing3_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing3_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing4_Hemin_chloride["Hemin chloride"]
CultureMech_009403 --> ing4_Hemin_chloride
CHEBI_50385["CHEBI:50385"]
ing4_Hemin_chloride -.-> CHEBI_50385
ing5_Triethanolamine["Triethanolamine"]
CultureMech_009403 --> ing5_Triethanolamine
CHEBI_28621["CHEBI:28621"]
ing5_Triethanolamine -.-> CHEBI_28621
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
197.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 1.0, 100.0, 96.0] |
| 2 | Lactobacilli MRS broth (BD-Difco) | 55 G_PER_L | Properties: Undefined component | |
| 3 |
Maltose
C12H22O11 342.297 g/mol
|
15 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 4 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 5 |
Hemin chloride
C34H32ClFeN4O4 651.952 g/mol
|
0.1 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Defined component, Simple component, Organic compound |
| 6 |
Triethanolamine
C6H15NO3 149.19 g/mol
|
4 G_PER_L |
CHEBI:28621
triethanolamine
|
Role: Solvating media; Properties: Defined component, Simple component, Organic compound |
Solutions
Maltose--hemin solution
Dose: 18 G_PER_L
Hemin solution (see below)
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.766560Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M286 |
| 2026-01-28T19:48:09.778900Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:15.853690+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:43.129743Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:06.051512Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009403 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.995843+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Lactobacillus Pontis Medium" to "lactobacillus_pontis_medium" for consistent matching |
| 2026-05-16T09:03:16.196842+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:39.464181+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |