Overview
- External ontology
- TOGO:M2872 — PYE Solid medium
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2872 imports PYE Solid medium from Marks et al. 2010. The article defines rich PYE as 0.2% Bacto peptone, 0.1% yeast extract, 1 mM MgSO4, and 0.5 mM CaCl2, then defines solid medium as PYE with 1.5% Bacto agar.
Composition
flowchart LR
CultureMech_009406["pye_solid_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_009406 --> ing0_Bacto_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_009406 --> ing1_Yeast_extract
ing2_MgSO4["MgSO4"]
CultureMech_009406 --> ing2_MgSO4
CHEBI_32599["CHEBI:32599"]
ing2_MgSO4 -.-> CHEBI_32599
ing3_CaCl2["CaCl2"]
CultureMech_009406 --> ing3_CaCl2
CHEBI_3312["CHEBI:3312"]
ing3_CaCl2 -.-> CHEBI_3312
ing4_Agar["Agar"]
CultureMech_009406 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 0.2 PERCENT_W_V |
MICRO:0000178 (source link unavailable)
Bacto peptone
|
Marks et al. 2010 lists 0.2% Bacto peptone in rich PYE. |
| 2 | Yeast extract | 0.1 PERCENT_W_V |
FOODON:03315426
Yeast extract
|
Marks et al. 2010 lists 0.1% yeast extract in rich PYE. |
| 3 |
MgSO4
Mg.O4S 120.368 g/mol
|
1 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
Marks et al. 2010 lists 1 mM MgSO4 in rich PYE. |
| 4 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.5 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
Marks et al. 2010 lists 0.5 mM CaCl2 in rich PYE. |
| 5 | Agar | 1.5 PERCENT_W_V |
CHEBI:2509
agar
|
Marks et al. 2010 lists PYE solid medium as PYE with 1.5% Bacto agar; the source-disclosed agar solidifier is grounded to agar. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.364952Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2872 |
| 2026-01-28T19:48:33.151286Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.060558Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009406 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.006455+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYE Solid medium" to "pye_solid_medium" for consistent matching |
| 2026-06-05T07:41:42.985424+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.568686+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m2872_pye_solid_score15.py | RESOLVED_TOGO_M2872_PYE_SOLID_SCORE15 | Grounded all five source-listed PYE Solid components to MediaIngredientMech, FoodOn, or ChEBI terms using TOGO M2872 and Marks et al. 2010. Left pH and recipe temperature unset because the source does not report a formulation pH and reports 30 C as a study incubation condition. |