Overview
- External ontology
- TOGO:M2878 — de Man, Rogosa, and Sharpe (MRS) broth (Sigma)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2878
Composition
flowchart LR
CultureMech_009412["de_man_rogosa_and_sharpe_mrs_broth_sigma"]
ing0_Distilled_water["Distilled water"]
CultureMech_009412 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MRS_broth__Sigma_["MRS broth (Sigma)"]
CultureMech_009412 --> ing1_MRS_broth__Sigma_
ing2_Magnesium_sulfate_heptahydrate["Magnesium sulfate heptahydrate"]
CultureMech_009412 --> ing2_Magnesium_sulfate_heptahydrate
CHEBI_31795["CHEBI:31795"]
ing2_Magnesium_sulfate_heptahydrate -.-> CHEBI_31795
ing3_Yeast_extract["Yeast extract"]
CultureMech_009412 --> ing3_Yeast_extract
ing4_Dipotassium_hydrogen_phosphate["Dipotassium hydrogen phosphate"]
CultureMech_009412 --> ing4_Dipotassium_hydrogen_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Dipotassium_hydrogen_phosphate -.-> CHEBI_131527
ing5_Manganous_sulfate_tetrahydrate["Manganous sulfate tetrahydrate"]
CultureMech_009412 --> ing5_Manganous_sulfate_tetrahydrate
ing6_Triammonium_citrate["Triammonium citrate"]
CultureMech_009412 --> ing6_Triammonium_citrate
CHEBI_63037["CHEBI:63037"]
ing6_Triammonium_citrate -.-> CHEBI_63037
ing7_Sodium_acetate_trihydrate["Sodium acetate trihydrate"]
CultureMech_009412 --> ing7_Sodium_acetate_trihydrate
CHEBI_32138["CHEBI:32138"]
ing7_Sodium_acetate_trihydrate -.-> CHEBI_32138
ing8_Glucose["Glucose"]
CultureMech_009412 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Meat_extract["Meat extract"]
CultureMech_009412 --> ing9_Meat_extract
ing10_Peptone["Peptone"]
CultureMech_009412 --> ing10_Peptone
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | MRS broth (Sigma) | 51 G_PER_L | ||
| 3 |
Magnesium sulfate heptahydrate
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 | Yeast extract | 4 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
Dipotassium hydrogen phosphate
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 | Manganous sulfate tetrahydrate | 0.05 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component | |
| 7 |
Triammonium citrate
C6H5O7.3H4N 243.216 g/mol
|
2 G_PER_L |
CHEBI:63037
triammonium citrate
|
Role: Nitrogen source; Properties: Simple component, Organic compound, Defined component |
| 8 |
Sodium acetate trihydrate
C2H3O2.3H2O.Na 136.079 g/mol
|
5 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 10 | Meat extract | 8 G_PER_L | Properties: Undefined component | |
| 11 | Peptone | 10 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.371952Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2878 |
| 2026-01-28T19:48:28.772772Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.089340Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009412 |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.037826+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "de Man, Rogosa, and Sharpe (MRS) broth (Sigma)" to "de_man_rogosa_and_sharpe_mrs_broth_sigma" for consistent matching |
| 2026-06-05T07:40:55.346556+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |