glucose_streptococcal_broth_medium

CultureMech:009419 bacterial COMPLEX LIQUID
Original name: Glucose streptococcal broth medium

Overview

External ontology
TOGO:M2884 — Glucose streptococcal broth medium
Applications
  • Microbial cultivation
Notes
TOGO M2884 lists glucose streptococcal broth as 1 L distilled water, 5 g yeast extract, 7.2 g Sodium-beta-glycerophosphate, 10 g glucose, 10 g meat extract, 5 g tryptose, and 5 g tryptone. TOGO cites Arendt et al. 1994 for Lactococcus lactis strains grown at 30 C in M17 medium supplemented with glucose or glucose streptococcal broth.

Composition

flowchart LR
    CultureMech_009419["glucose_streptococcal_broth_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009419 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_009419 --> ing1_Yeast_extract
    ing2_Sodium_beta_glycerophosphate["Sodium beta-glycerophosphate"]
    CultureMech_009419 --> ing2_Sodium_beta_glycerophosphate
    CHEBI_132089["CHEBI:132089"]
    ing2_Sodium_beta_glycerophosphate -.-> CHEBI_132089
    ing3_Glucose["Glucose"]
    CultureMech_009419 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Meat_extract["Meat extract"]
    CultureMech_009419 --> ing4_Meat_extract
    ing5_Tryptose["Tryptose"]
    CultureMech_009419 --> ing5_Tryptose
    ing6_Tryptone["Tryptone"]
    CultureMech_009419 --> ing6_Tryptone

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M2884 lists 1 L distilled water.
2 Yeast extract 5.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M2884 lists 5 g/L yeast extract.
3 Sodium beta-glycerophosphate
C3H7O6P.2Na 216.037 g/mol
7.2 G_PER_L CHEBI:132089
sodium glycerol 2-phosphate
 TOGO M2884 lists 7.2 g/L Sodium-beta-glycerophosphate.
4 Glucose
C6H12O6 180.156 g/mol
10.0 G_PER_L CHEBI:17234
glucose
 TOGO M2884 lists 10 g/L glucose.
5 Meat extract 10.0 G_PER_L TOGO M2884 lists 10 g/L meat extract; this biological extract is retained as an opaque complex component.
6 Tryptose 5.0 G_PER_L TOGO M2884 lists 5 g/L tryptose; this Bacto Tryptose-like peptone product is retained as an opaque complex component.
7 Tryptone 5.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 TOGO M2884 lists 5 g/L tryptone.

Preparation

  1. Dissolve 5.0 g yeast extract, 7.2 g sodium beta-glycerophosphate, 10.0 g glucose, 10.0 g meat extract, 5.0 g tryptose, and 5.0 g tryptone in 1.0 L distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.378164Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2884
2026-01-28T19:48:40.138229Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:06.114610Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009419
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.065451+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Glucose streptococcal broth medium" to "glucose_streptococcal_broth_medium" for consistent matching
2026-06-05T07:41:06.497627+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2884_score15.py RESOLVED_TOGO_M2884_SCORE15 TOGO M2884 lists glucose streptococcal broth as 1 L distilled water, 5 g yeast extract, 7.2 g Sodium-beta-glycerophosphate, 10 g glucose, 10 g meat extract, 5 g tryptose, and 5 g tryptone. TOGO cites Arendt et al. 1994 for Lactococcus lactis strains grown at 30 C in M17 medium supplemented with glucose or glucose streptococcal broth. Corrected the imported distilled-water unit from g/L to 1 L and grounded water, yeast extract, sodium beta-glycerophosphate, glucose, and tryptone.