modified_pleuropneumonia_like_organism_pplo_medium (CultureMech:009422)

Overview

External ontology

TOGO:M2887 — Modified pleuropneumonia-like organism (PPLO) medium

Applications

Microbial cultivation

Notes

TOGO M2887 reports that Mycoplasma was cultured in modified pleuropneumonia-like organism medium supplemented with 20% inactivated horse serum from Hyclone, 10% yeast extract, thallium acetate at 0.125 mg/ml, and penicillin at 200 IU/ml.

Composition

flowchart LR
    CultureMech_009422["modified_pleuropneumonia_like_organism_pplo_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_009422 --> ing0_Yeast_extract
    ing1_Inactivated_horse_serum__Hyclone_["Inactivated horse serum (Hyclone)"]
    CultureMech_009422 --> ing1_Inactivated_horse_serum__Hyclone_
    ing2_Thallium_acetate["Thallium acetate"]
    CultureMech_009422 --> ing2_Thallium_acetate
    CHEBI_75192["CHEBI:75192"]
    ing2_Thallium_acetate -.-> CHEBI_75192
    ing3_PPLO_medium["PPLO medium"]
    CultureMech_009422 --> ing3_PPLO_medium
    ing4_Penicillin["Penicillin"]
    CultureMech_009422 --> ing4_Penicillin
    CHEBI_17334["CHEBI:17334"]
    ing4_Penicillin -.-> CHEBI_17334

Ingredients (5)

#	Ingredient	Concentration	Ontology	Notes
1	Yeast extract	10.0 PERCENT_W_V	FOODON:03315426 yeast extract	TOGO M2887 lists 10% Yeast extract.
2	Inactivated horse serum (Hyclone)	20.0 PERCENT_V_V		TOGO M2887 lists 20% Inactivated horse serum from Hyclone; this serum product is retained as an opaque component.
3	Thallium acetate C2H3O2.Tl 263.427 g/mol	0.125 G_PER_L	CHEBI:75192 thallium(I) acetate	TOGO M2887 lists 0.125 mg/ml Thallium acetate, equivalent to 0.125 g/L.
4	PPLO medium	1.0 L		TOGO M2887 lists 1 L PPLO medium; this undefined complex medium base is retained as an opaque component.
5	Penicillin C9H11N2O4SR 243.26 g/mol	variable VARIABLE	CHEBI:17334 penicillin	TOGO M2887 lists 200 IU/ml Penicillin, which is retained as a variable concentration because the schema has no unit for activity units.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.381634Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2887
2026-01-28T19:48:26.802884Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Penicillin
2026-03-13T06:20:06.127926Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009422
2026-03-17T06:30:22.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.079633+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified pleuropneumonia-like organism (PPLO) medium" to "modified_pleuropneumonia_like_organism_pplo_medium" for consistent matching
2026-05-16T09:04:26.145008+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:41:37.625408+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00	repair_togo_m2887_modified_pplo_score15.py	RESOLVED_TOGO_M2887_SCORE15	TOGO M2887 reports that Mycoplasma was cultured in modified pleuropneumonia-like organism medium supplemented with 20% inactivated horse serum from Hyclone, 10% yeast extract, thallium acetate at 0.125 mg/ml, and penicillin at 200 IU/ml. Corrected the imported 1 L PPLO medium unit, normalized the horse serum concentration as v/v, grounded thallium acetate, and retained Penicillin as an activity-unit amount.