Overview
- External ontology
- TOGO:M2889 — Brain-heart-infusion media (BHI) supplemented with 0.5% of Tween 80
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2889 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009424["brain_heart_infusion_media_bhi_supplemented_with_0_5_of_tween_80"]
ing0_Distilled_water["Distilled water"]
CultureMech_009424 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Tween_80["Tween 80"]
CultureMech_009424 --> ing1_Tween_80
CHEBI_53426["CHEBI:53426"]
ing1_Tween_80 -.-> CHEBI_53426
ing2_Calf_brains["Calf brains"]
CultureMech_009424 --> ing2_Calf_brains
ing3_Beef_heart["Beef heart"]
CultureMech_009424 --> ing3_Beef_heart
ing4_Proteose_peptone["Proteose peptone"]
CultureMech_009424 --> ing4_Proteose_peptone
ing5_Dextrose["Dextrose"]
CultureMech_009424 --> ing5_Dextrose
CHEBI_17634["CHEBI:17634"]
ing5_Dextrose -.-> CHEBI_17634
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_009424 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Disodium_phosphate["Disodium phosphate"]
CultureMech_009424 --> ing7_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing7_Disodium_phosphate -.-> CHEBI_34683
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.5 PERCENT_W_V |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Simple component, Organic compound |
| 3 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 4 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 5 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 6 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 8 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.383794Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2889 |
| 2026-01-28T19:48:14.003100Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.135767Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009424 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.088015+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Brain-heart-infusion media (BHI) supplemented with 0.5% of Tween 80" to "brain_heart_infusion_media_bhi_supplemented_with_0_5_of_tween_80" for consistent matching |
| 2026-06-05T07:40:51.526924+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |