complete_pleuropneumonia_like_organism_pplo_medium

CultureMech:009427 bacterial COMPLEX LIQUID
Original name: Complete pleuropneumonia-like organism (PPLO) medium

Overview

External ontology
TOGO:M2891 — Complete pleuropneumonia-like organism (PPLO) medium
Applications
  • Microbial cultivation
Notes
TOGO M2891 lists one liter of PPLO medium and approximately 48 h cultivation at 37 C in 5% CO2.

Composition

flowchart LR
    CultureMech_009427["complete_pleuropneumonia_like_organism_pplo_medium"]
    ing0_PPLO_medium["PPLO medium"]
    CultureMech_009427 --> ing0_PPLO_medium
    ing1_CO2["CO2"]
    CultureMech_009427 --> ing1_CO2
    CHEBI_16526["CHEBI:16526"]
    ing1_CO2 -.-> CHEBI_16526

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 PPLO medium 1000 ML_PER_L TOGO M2891 lists 1000 ML_PER_L of PPLO medium.
2 CO2
CO2 44.009 g/mol
5 PERCENT_V_V CHEBI:16526
carbon dioxide
TOGO M2891 lists 5% CO2 as the incubation atmosphere.

Preparation

  1. Use one liter of PPLO medium.
  2. Cultivate for approximately 48 h at 37 C in 5% CO2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.385457Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2891
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO2
2026-03-13T06:20:06.153521Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009427
2026-03-17T19:46:25.106476+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Complete pleuropneumonia-like organism (PPLO) medium" to "complete_pleuropneumonia_like_organism_pplo_medium" for consistent matching
2026-05-16T09:03:28.237126+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-09-10T00:00:00-07:00 repair_togo_condition_wrappers_score20.py RESOLVED_TOGO_CONDITION_WRAPPERS_SCORE20 TOGO M2891 lists one liter of PPLO medium and approximately 48 h cultivation at 37 C in 5% CO2.