Overview
- External ontology
- TOGO:M2894 — Nutrient broth (NB II)
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2894 records Nutrient broth (NB II) with 3.5 g/L peptone from casein, 2.5 g/L peptone from meat, 2.5 g/L peptone from gelatine, 1.5 g/L yeast extract, 5 g/L NaCl, and incubation at 30 C.
Composition
flowchart LR
CultureMech_009430["nutrient_broth_nb_ii"]
ing0_Peptone_from_casein["Peptone from casein"]
CultureMech_009430 --> ing0_Peptone_from_casein
ing1_Peptone_from_meat["Peptone from meat"]
CultureMech_009430 --> ing1_Peptone_from_meat
ing2_Peptone_from_gelatine["Peptone from gelatine"]
CultureMech_009430 --> ing2_Peptone_from_gelatine
ing3_Yeast_extract["Yeast extract"]
CultureMech_009430 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_009430 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Distilled_water["Distilled water"]
CultureMech_009430 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone from casein | 3.5 G_PER_L |
FOODON:03315719
Casein peptone
|
TOGO M2894 lists 3.5 g/L peptone from casein. |
| 2 | Peptone from meat | 2.5 G_PER_L | TOGO M2894 lists 2.5 g/L peptone from meat; the source-specific meat hydrolysate is retained without an ontology grounding. | |
| 3 | Peptone from gelatine | 2.5 G_PER_L | TOGO M2894 lists 2.5 g/L peptone from gelatine; the source-specific gelatin hydrolysate is retained without an ontology grounding. | |
| 4 | Yeast extract | 1.5 G_PER_L |
FOODON:03315426
Yeast extract
|
TOGO M2894 lists 1.5 g/L Yeast extract. |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
TOGO M2894 lists 5.0 g/L NaCl. |
| 6 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M2894 records the NB II formulation per 1 L Distilled water. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.387722Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2894 |
| 2026-01-28T19:48:34.926530Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.162865Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009430 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.121730+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient broth (NB II)" to "nutrient_broth_nb_ii" for consistent matching |
| 2026-06-05T07:41:40.180490+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_nutrient_broths_score15.py | RESOLVED_TOGO_M2894_NBII | TOGO M2894 records Nutrient broth (NB II) with 3.5 g/L peptone from casein, 2.5 g/L peptone from meat, 2.5 g/L peptone from gelatine, 1.5 g/L yeast extract, 5 g/L NaCl, and incubation at 30 C. |