Overview
- External ontology
- TOGO:M2906 — Man, Rogosa, and Sharpe (MRS) medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2906
Composition
flowchart LR
CultureMech_009444["man_rogosa_and_sharpe_mrs_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009444 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009444 --> ing1_Yeast_extract
ing2_Dipotassium_hydrogen_phosphate["Dipotassium hydrogen phosphate"]
CultureMech_009444 --> ing2_Dipotassium_hydrogen_phosphate
CHEBI_131527["CHEBI:131527"]
ing2_Dipotassium_hydrogen_phosphate -.-> CHEBI_131527
ing3_Sodium_acetate["Sodium acetate"]
CultureMech_009444 --> ing3_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Sodium_acetate -.-> CHEBI_32954
ing4_Tween_80["Tween 80"]
CultureMech_009444 --> ing4_Tween_80
CHEBI_53426["CHEBI:53426"]
ing4_Tween_80 -.-> CHEBI_53426
ing5_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_009444 --> ing5_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing5_Magnesium_sulfate -.-> CHEBI_32599
ing6_Triammonium_citrate["Triammonium citrate"]
CultureMech_009444 --> ing6_Triammonium_citrate
CHEBI_63037["CHEBI:63037"]
ing6_Triammonium_citrate -.-> CHEBI_63037
ing7_Manganese_sulfate["Manganese sulfate"]
CultureMech_009444 --> ing7_Manganese_sulfate
CHEBI_86364["CHEBI:86364"]
ing7_Manganese_sulfate -.-> CHEBI_86364
ing8_Glucose["Glucose"]
CultureMech_009444 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Beef_extract["Beef extract"]
CultureMech_009444 --> ing9_Beef_extract
ing10_Peptone["Peptone"]
CultureMech_009444 --> ing10_Peptone
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 4 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Dipotassium hydrogen phosphate
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 5 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 6 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.2 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Triammonium citrate
C6H5O7.3H4N 243.216 g/mol
|
2 G_PER_L |
CHEBI:63037
triammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component |
| 8 |
Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.05 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 10 | Beef extract | 10 G_PER_L | Properties: Undefined component | |
| 11 | Peptone | 10 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.397725Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2906 |
| 2026-01-28T19:48:40.873137Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.211011Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009444 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.180697+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Man, Rogosa, and Sharpe (MRS) medium" to "man_rogosa_and_sharpe_mrs_medium" for consistent matching |
| 2026-06-05T07:41:12.663453+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |