Overview
- External ontology
- TOGO:M290 — EGGC Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M290 Original source: JCM - JCM_M296 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=296
Composition
flowchart LR
CultureMech_009446["eggc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009446 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009446 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009446 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_009446 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_009446 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_Sodium_acetate["Sodium acetate"]
CultureMech_009446 --> ing5_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Sodium_acetate -.-> CHEBI_32954
ing6_CaCO3["CaCO3"]
CultureMech_009446 --> ing6_CaCO3
CHEBI_3311["CHEBI:3311"]
ing6_CaCO3 -.-> CHEBI_3311
ing7_Glucose["Glucose"]
CultureMech_009446 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_Agar["Agar"]
CultureMech_009446 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_009446 --> ing9_Casamino_acids__BD_Difco_
ing10_0_5_M_Na2HPO4__NaH2PO4_buffer__pH_7_3_["0.5 M Na2HPO4--NaH2PO4 buffer (pH 7.3)"]
CultureMech_009446 --> ing10_0_5_M_Na2HPO4__NaH2PO4_buffer__pH_7_3_
ing11_Biotin["Biotin"]
CultureMech_009446 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_009446 --> ing12_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p__Aminobenzoic_acid -.-> CHEBI_30753
ing13_Thiamine_HCl["Thiamine・HCl"]
CultureMech_009446 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009446 --> ing14_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing14_Calcium_pantothenate -.-> CHEBI_31345
ing15_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_009446 --> ing15_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_HCl -.-> CHEBI_30961
ing16_Folic_acid["Folic acid"]
CultureMech_009446 --> ing16_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing16_Folic_acid -.-> CHEBI_27470
ing17_Vitamin_B12["Vitamin B12"]
CultureMech_009446 --> ing17_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing17_Vitamin_B12 -.-> CHEBI_176843
ing18_Riboflavin["Riboflavin"]
CultureMech_009446 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_009446 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_myo__Inositol["myo--Inositol"]
CultureMech_009446 --> ing20_myo__Inositol
CHEBI_17268["CHEBI:17268"]
ing20_myo__Inositol -.-> CHEBI_17268
ing21_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_009446 --> ing21_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing21_FeCl3_6H2O -.-> CHEBI_86254
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
302.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 300.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.05 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.3 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 7 |
CaCO3
CO3.Ca 100.086 g/mol
|
0.02 G_PER_L |
CHEBI:3311
calcium carbonate
|
Role: Protective agent, Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
0.6 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 9 | Agar | 12 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 10 | Casamino acids (BD-Difco) | 0.3 G_PER_L | Properties: Undefined component | |
| 11 | 0.5 M Na2HPO4--NaH2PO4 buffer (pH 7.3) | 2 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Inorganic compound, Complex component | |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
20 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
20 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
20 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
20 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Folic acid
C19H19N7O6 441.404 g/mol
|
1 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
20 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
20 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
myo--Inositol
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17268
myo-inositol
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 22 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.15 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A (see below)
Dose: 4.5 G_PER_L
Vitamins mix solution (see below)
Dose: 0.5 G_PER_L
FeCl3 solution (see below)
Dose: 2 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.775016Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M290 |
| 2026-01-28T19:48:24.380114Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:40.284906+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:10.228460Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:06.224441Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009446 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.194648+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EGGC Medium" to "eggc_medium" for consistent matching |
| 2026-05-16T09:03:16.233102+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:39.502303+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:33.753382+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:28.917510+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.996921+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |