Overview
- External ontology
- TOGO:M2922 — LBS medium
- Applications
-
- Microbial cultivation
- pH
- 7.5
- High-metal flag
- True
- Notes
- TOGO M2922 reports LBS medium with 1000 ml/L Distilled water, 1.5 g/L Yeast extract, 342 mM NaCl, 20 mM Tris at pH 7.5, and 10 g/L Tryptone.
Composition
flowchart LR
CultureMech_009458["lbs_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009458 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009458 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009458 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Tris__pH_7_5["Tris, pH 7.5"]
CultureMech_009458 --> ing3_Tris__pH_7_5
CHEBI_9754["CHEBI:9754"]
ing3_Tris__pH_7_5 -.-> CHEBI_9754
ing4_Tryptone["Tryptone"]
CultureMech_009458 --> ing4_Tryptone
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
TOGO M2922 lists 1000 ml/L Distilled water. |
| 2 | Yeast extract | 1.5 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M2922 lists 1.5 g/L Yeast extract. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
342 MILLIMOLAR |
CHEBI:26710
sodium chloride
|
TOGO M2922 lists 342 mM NaCl. |
| 4 |
Tris, pH 7.5
C4H11NO3 121.136 g/mol
|
20 MILLIMOLAR |
CHEBI:9754
tris
|
TOGO M2922 lists 20 mM Tris, pH 7.5. |
| 5 | Tryptone | 10 G_PER_L | TOGO M2922 lists 10 g/L Tryptone as a digest product not reducible to one ChEBI molecule. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.406314Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2922 |
| 2026-01-28T19:48:40.783118Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.257544Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009458 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.231929+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LBS medium" to "lbs_medium" for consistent matching |
| 2026-06-05T07:41:10.960147+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m2922_lbs_score15.py | RESOLVED_TOGO_M2922_LBS_SCORE15 | TOGO M2922 reports LBS medium with 1000 ml/L Distilled water, 1.5 g/L Yeast extract, 342 mM NaCl, 20 mM Tris at pH 7.5, and 10 g/L Tryptone. Corrected the imported distilled-water row from a g/L artifact to 1000 ml/L and added explicit FoodOn/ChEBI grounding for the four mappable components. |