Overview
- External ontology
- TOGO:M2923 — Brain–heart infusion (BHI) broth supplemented with haemin and NAD
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2923 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009459["brain_heart_infusion_bhi_broth_supplemented_with_haemin_and_nad"]
ing0_Haemin["Haemin"]
CultureMech_009459 --> ing0_Haemin
CHEBI_50385["CHEBI:50385"]
ing0_Haemin -.-> CHEBI_50385
ing1_NAD["NAD"]
CultureMech_009459 --> ing1_NAD
ing2_Calf_brains["Calf brains"]
CultureMech_009459 --> ing2_Calf_brains
ing3_Beef_heart["Beef heart"]
CultureMech_009459 --> ing3_Beef_heart
ing4_Proteose_peptone["Proteose peptone"]
CultureMech_009459 --> ing4_Proteose_peptone
ing5_Dextrose["Dextrose"]
CultureMech_009459 --> ing5_Dextrose
CHEBI_17634["CHEBI:17634"]
ing5_Dextrose -.-> CHEBI_17634
ing6_Sodium_chloride["Sodium chloride"]
CultureMech_009459 --> ing6_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing6_Sodium_chloride -.-> CHEBI_26710
ing7_Disodium_phosphate["Disodium phosphate"]
CultureMech_009459 --> ing7_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing7_Disodium_phosphate -.-> CHEBI_34683
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
10 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Defined component, Organic compound, Simple component |
| 2 | NAD | 2 G_PER_L | Role: Growth factor; Properties: Defined component, Simple component, Organic compound | |
| 3 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 4 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 5 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 6 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 7 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 8 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.407232Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2923 |
| 2026-01-28T19:48:19.514343Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.260574Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009459 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.235504+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Brain–heart infusion (BHI) broth supplemented with haemin and NAD" to "brain_heart_infusion_bhi_broth_supplemented_with_haemin_and_nad" for consistent matching |
| 2026-06-05T07:40:51.468218+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |