Overview
- External ontology
- TOGO:M2929 — Mueller-Hinton medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2929 Commercial Product: Mueller-Hinton Agar Supplier: Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Catalog: BD 211443, Sigma 70191 Source: https://microbenotes.com/mueller-hinton-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009463["mueller_hinton_medium"]
ing0_Beef_extract["Beef extract"]
CultureMech_009463 --> ing0_Beef_extract
ing1_Acid_hydrolysate_of_casein["Acid hydrolysate of casein"]
CultureMech_009463 --> ing1_Acid_hydrolysate_of_casein
ing2_Starch["Starch"]
CultureMech_009463 --> ing2_Starch
CHEBI_28017["CHEBI:28017"]
ing2_Starch -.-> CHEBI_28017
ing3_Agar["Agar"]
CultureMech_009463 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009463 --> ing4_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing4_Carbon_dioxide_gas -.-> CHEBI_16526
ing5_Nitrogen_gas["Nitrogen gas"]
CultureMech_009463 --> ing5_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing5_Nitrogen_gas -.-> CHEBI_17997
ing6_Oxygen_gas["Oxygen gas"]
CultureMech_009463 --> ing6_Oxygen_gas
ing7_Hydrogen_gas["Hydrogen gas"]
CultureMech_009463 --> ing7_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing7_Hydrogen_gas -.-> CHEBI_18276
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Beef extract
Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Mueller-Hinton Agar BD 211443, Sigma 70191
|
2.0 G_PER_L |
FOODON:03302088
beef extract
|
Aqueous beef extract providing nutrients and growth factors; Constituent of Mueller-Hinton Agar commercial product |
| 2 |
Acid hydrolysate of casein
Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Mueller-Hinton Agar BD 211443, Sigma 70191
|
17.5 G_PER_L |
MICRO:0001366 (source link unavailable)
casein hydrolysate
|
Casein enzymatic hydrolysate, provides amino acids and peptides. Low PABA and thymine content critical for sulfonamide/trimethoprim testing; Constituent of Mueller-Hinton Agar commercial product |
| 3 |
Starch
Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Mueller-Hinton Agar BD 211443, Sigma 70191
|
1.5 G_PER_L |
CHEBI:28017
starch
|
Acts as protective colloid and absorbs toxic metabolites released from bacteria; Constituent of Mueller-Hinton Agar commercial product |
| 4 |
Agar
Multiple suppliers (BD, Sigma-Aldrich, HiMedia) Mueller-Hinton Agar BD 211443, Sigma 70191
|
17.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent; Constituent of Mueller-Hinton Agar commercial product |
| 5 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 6 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 7 | Oxygen gas | variable VARIABLE | Properties: Gas | |
| 8 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.412324Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2929 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-13T06:20:06.280060Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009463 |
| 2026-03-17T19:46:25.255379+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mueller-Hinton medium" to "mueller_hinton_medium" for consistent matching |
| 2026-05-16T09:04:27.044429+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-05-17T07:07:33.002963+00:00 | cleanup_residual_errors.py | CLEANUP_RESIDUAL_ERRORS | R01_flatten_synonym_text=2 |