Overview
- External ontology
- TOGO:M2932 — Brain Heart Infusion agar (amended with 5% (v/v) laked horse blood)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2932 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009466["brain_heart_infusion_agar_amended_with_5_v_v_laked_horse_blood"]
ing0_laked_horse_blood__Hema_Resource___Supply__Aurora__OR_["laked horse blood (Hema Resource & Supply, Aurora, OR)"]
CultureMech_009466 --> ing0_laked_horse_blood__Hema_Resource___Supply__Aurora__OR_
ing1_Calf_brains["Calf brains"]
CultureMech_009466 --> ing1_Calf_brains
ing2_Beef_heart["Beef heart"]
CultureMech_009466 --> ing2_Beef_heart
ing3_Proteose_peptone["Proteose peptone"]
CultureMech_009466 --> ing3_Proteose_peptone
ing4_Dextrose["Dextrose"]
CultureMech_009466 --> ing4_Dextrose
CHEBI_17634["CHEBI:17634"]
ing4_Dextrose -.-> CHEBI_17634
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_009466 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Disodium_phosphate["Disodium phosphate"]
CultureMech_009466 --> ing6_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing6_Disodium_phosphate -.-> CHEBI_34683
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009466 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_009466 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
ing9_Hydrogen_gas["Hydrogen gas"]
CultureMech_009466 --> ing9_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing9_Hydrogen_gas -.-> CHEBI_18276
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | laked horse blood (Hema Resource & Supply, Aurora, OR) | 5 G_PER_L | Role: Nutrient source | |
| 2 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 4 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 5 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 7 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 10 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.413636Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2932 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-13T06:20:06.295341Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009466 |
| 2026-03-17T19:46:25.270806+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Brain Heart Infusion agar (amended with 5% (v/v) laked horse blood)" to "brain_heart_infusion_agar_amended_with_5_v_v_laked_horse_blood" for consistent matching |
| 2026-05-16T09:03:26.075111+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |