Overview
- External ontology
- TOGO:M2966 — modified Luria broth
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2966 extracts the modified Luria broth formulation used by Mobley et al. 1990: per liter, 10 g tryptone from Difco, 5 g yeast extract, 8.5 g NaCl, and 100 mM HEPES at pH 7.2, diluted to 300 mosmol.
Composition
flowchart LR
CultureMech_009490["modified_luria_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_009490 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_yeast_extract["yeast extract"]
CultureMech_009490 --> ing1_yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009490 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_HEPES["HEPES"]
CultureMech_009490 --> ing3_HEPES
CHEBI_42334["CHEBI:42334"]
ing3_HEPES -.-> CHEBI_42334
ing4_tryptone__Difco_["tryptone [Difco]"]
CultureMech_009490 --> ing4_tryptone__Difco_
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
TOGO M2966 records the formulation with 1 L Distilled water. |
| 2 | yeast extract | 5.0 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M2966 / Mobley et al. 1990 lists 5 g/L yeast extract. |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
8.5 G_PER_L |
CHEBI:26710
sodium chloride
|
TOGO M2966 / Mobley et al. 1990 lists 8.5 g/L NaCl. |
| 4 |
HEPES
C8H18N2O4S 238.309 g/mol
|
100.0 MILLIMOLAR |
CHEBI:42334
HEPES
|
TOGO M2966 / Mobley et al. 1990 lists 100 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid [HEPES; pH 7.2]. |
| 5 | tryptone [Difco] | 10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
TOGO M2966 / Mobley et al. 1990 lists 10 g/L tryptone from Difco. |
Preparation
- Prepare the modified Luria broth from 1 L distilled water, 10 g/L tryptone, 5 g/L yeast extract, 8.5 g/L NaCl, and 100 mM HEPES at pH 7.2.
- Dilute the medium to 300 mosmol.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.430962Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2966 |
| 2026-01-28T19:48:10.123558Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.379731Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009490 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.364884+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "modified Luria broth" to "modified_luria_broth" for consistent matching |
| 2026-06-05T07:41:36.800859+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m2966_modified_luria_score15.py | RESOLVED_TOGO_M2966_SCORE15 | TOGO M2966 extracts the modified Luria broth formulation used by Mobley et al. 1990: per liter, 10 g tryptone from Difco, 5 g yeast extract, 8.5 g NaCl, and 100 mM HEPES at pH 7.2, diluted to 300 mosmol. Corrected the imported 1 L water row from grams per liter to liters and grounded yeast extract, tryptone, and HEPES. |