Overview
- External ontology
- TOGO:M2969 — brain heart infusion medium (with added NAD and hemin)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2969 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009491["brain_heart_infusion_medium_with_added_nad_and_hemin"]
ing0_Calf_brains["Calf brains"]
CultureMech_009491 --> ing0_Calf_brains
ing1_Beef_heart["Beef heart"]
CultureMech_009491 --> ing1_Beef_heart
ing2_Proteose_peptone["Proteose peptone"]
CultureMech_009491 --> ing2_Proteose_peptone
ing3_Dextrose["Dextrose"]
CultureMech_009491 --> ing3_Dextrose
CHEBI_17634["CHEBI:17634"]
ing3_Dextrose -.-> CHEBI_17634
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_009491 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Disodium_phosphate["Disodium phosphate"]
CultureMech_009491 --> ing5_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing5_Disodium_phosphate -.-> CHEBI_34683
ing6_hemin__ICN_["hemin (ICN)"]
CultureMech_009491 --> ing6_hemin__ICN_
ing7_NAD__Sigma_["NAD (Sigma)"]
CultureMech_009491 --> ing7_NAD__Sigma_
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 2 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 4 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 6 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 7 | hemin (ICN) | variable VARIABLE | Role: Growth factor; Properties: Defined component, Organic compound, Simple component | |
| 8 | NAD (Sigma) | variable VARIABLE | Role: Growth factor; Properties: Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.431943Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2969 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: hemin (ICN); Added default concentration for ingredient: NAD (Sigma) |
| 2026-03-13T06:20:06.382683Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009491 |
| 2026-03-17T19:46:25.368106+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "brain heart infusion medium (with added NAD and hemin)" to "brain_heart_infusion_medium_with_added_nad_and_hemin" for consistent matching |
| 2026-05-16T09:03:26.206820+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |