brain_heart_infusion_broth_supplemented_with_0_1_tris_base_and_0_001_thiamine_monophosphate

CultureMech:009497 bacterial COMPLEX LIQUID
Original name: brain-heart infusion broth (supplemented with 0.1% Tris base and 0.001% thiamine monophosphate)

Overview

External ontology
TOGO:M2974 — brain-heart infusion broth (supplemented with 0.1% Tris base and 0.001% thiamine monophosphate)
Applications
  • Microbial cultivation
Notes
TOGO M2974 records BHI-TT as 1 L brain-heart infusion broth from Difco Laboratories supplemented with 0.1% Tris base and 0.001% thiamine monophosphate; the source does not spell out the commercial broth composition.

Composition

flowchart LR
    CultureMech_009497["brain_heart_infusion_broth_supplemented_with_0_1_tris_base_and_0_00..."]
    ing0_brain_heart_infusion_broth__Difco_Laboratories_["brain-heart infusion broth (Difco Laboratories)"]
    CultureMech_009497 --> ing0_brain_heart_infusion_broth__Difco_Laboratories_
    ing1_Tris["Tris"]
    CultureMech_009497 --> ing1_Tris
    CHEBI_9754["CHEBI:9754"]
    ing1_Tris -.-> CHEBI_9754
    ing2_thiamine_monophosphate["thiamine monophosphate"]
    CultureMech_009497 --> ing2_thiamine_monophosphate
    CHEBI_9533["CHEBI:9533"]
    ing2_thiamine_monophosphate -.-> CHEBI_9533

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 brain-heart infusion broth (Difco Laboratories) 1000.0 ML_PER_L TOGO M2974 lists 1 L prepared brain-heart infusion broth from Difco Laboratories; the source does not spell out the commercial broth composition.
2 Tris
C4H11NO3 121.136 g/mol
0.1 PERCENT_W_V CHEBI:9754
tris
 TOGO M2974 supplements BHI broth with 0.1% Tris base.
3 thiamine monophosphate
C12H18N4O4PS 345.341 g/mol
0.001 PERCENT_W_V CHEBI:9533
thiamine(1+) monophosphate
 TOGO M2974 supplements BHI broth with 0.001% thiamine monophosphate.

Preparation

  1. Supplement 1 L prepared brain-heart infusion broth with 0.1% Tris base and 0.001% thiamine monophosphate.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.435443Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2974
2026-01-28T19:48:34.652865Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:06.413807Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009497
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.414133+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "brain-heart infusion broth (supplemented with 0.1% Tris base and 0.001% thiamine monophosphate)" to "brain_heart_infusion_broth_supplemented_with_0_1_tris_base_and_0_001_thiamine_monophosphate" for consistent matching
2026-06-05T07:40:51.493800+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m2974_bhi_tt_score15.py RESOLVED_TOGO_M2974_BHI_TT_SCORE15 TOGO M2974 records BHI-TT as 1 L brain-heart infusion broth from Difco Laboratories supplemented with 0.1% Tris base and 0.001% thiamine monophosphate; the source does not spell out the commercial broth composition.