mineral_sulfide_free_medium

CultureMech:009510 bacterial COMPLEX LIQUID
Original name: mineral-sulfide-free medium

Overview

External ontology
TOGO:M2991 — mineral-sulfide-free medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2991

Composition

flowchart LR
    CultureMech_009510["mineral_sulfide_free_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009510 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4.7H2O"]
    CultureMech_009510 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_KH2PO4["KH2PO4"]
    CultureMech_009510 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_FeSO4_7H2O["FeSO4.7H2O"]
    CultureMech_009510 --> ing3_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing3_FeSO4_7H2O -.-> CHEBI_75836
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009510 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_meat_extract["meat extract"]
    CultureMech_009510 --> ing5_meat_extract
    ing6_K2S4O6["K2S4O6"]
    CultureMech_009510 --> ing6_K2S4O6
    CHEBI_86466["CHEBI:86466"]
    ing6_K2S4O6 -.-> CHEBI_86466

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 KH2PO4
H2O4P.K 136.084 g/mol
0.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
20 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.4 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
6 meat extract 1 G_PER_L Properties: Undefined component
7 K2S4O6
2K.O6S4 302.458 g/mol
4 G_PER_L CHEBI:86466
potassium tetrathionate
Properties: Defined component, Simple component, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.444761Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2991
2026-01-28T19:48:23.486171Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:06.471590Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009510
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.476323+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "mineral-sulfide-free medium" to "mineral_sulfide_free_medium" for consistent matching
2026-06-05T07:41:34.579380+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.044960+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.