Overview
- External ontology
- TOGO:M2993 — PPM
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2993 Original source: NBRC - NBRC_M1381-2 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1381
Composition
flowchart LR
CultureMech_009511["ppm"]
ing0_Distilled_water["Distilled water"]
CultureMech_009511 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009511 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_MES["MES"]
CultureMech_009511 --> ing2_MES
CHEBI_39010["CHEBI:39010"]
ing2_MES -.-> CHEBI_39010
ing3_NH4H2PO4["NH4H2PO4"]
CultureMech_009511 --> ing3_NH4H2PO4
CHEBI_62982["CHEBI:62982"]
ing3_NH4H2PO4 -.-> CHEBI_62982
ing4_Gelrite__if_needed_["Gelrite (if needed)"]
CultureMech_009511 --> ing4_Gelrite__if_needed_
ing5_Cycloheximide["Cycloheximide"]
CultureMech_009511 --> ing5_Cycloheximide
CHEBI_27641["CHEBI:27641"]
ing5_Cycloheximide -.-> CHEBI_27641
ing6_Pectin["Pectin"]
CultureMech_009511 --> ing6_Pectin
CHEBI_17309["CHEBI:17309"]
ing6_Pectin -.-> CHEBI_17309
ing7_Xylan_from_Beechwood["Xylan from Beechwood"]
CultureMech_009511 --> ing7_Xylan_from_Beechwood
CHEBI_15447["CHEBI:15447"]
ing7_Xylan_from_Beechwood -.-> CHEBI_15447
ing8_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_009511 --> ing8_Bacto_Yeast_Extract__Difco_
ing9_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
CultureMech_009511 --> ing9_Bacto_Casamino_Acids__Difco_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | MES | 2 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 4 |
NH4H2PO4
H2O4P.H4N 115.025 g/mol
|
0.053 G_PER_L |
CHEBI:62982
ammonium dihydrogen phosphate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 5 | Gelrite (if needed) | 8 G_PER_L | Role: Solidifying component; Properties: Undefined component, Organic compound | |
| 6 |
Cycloheximide
C15H23NO4 281.352 g/mol
|
0.1 G_PER_L |
CHEBI:27641
cycloheximide
|
Role: Antimicrobial agent; Properties: Simple component, Organic compound, Defined component |
| 7 | Pectin | 0.05 G_PER_L |
CHEBI:17309
pectin
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 8 |
Xylan from Beechwood
(C5H8O4)n.H2O 150.13 g/mol
|
0.05 G_PER_L |
CHEBI:15447
(1->4)-beta-D-xylan
|
Role: Carbon source; Properties: Undefined component |
| 9 | Bacto Yeast Extract (Difco) | 0.05 G_PER_L | Properties: Undefined component | |
| 10 | Bacto Casamino Acids (Difco) | 0.05 G_PER_L | Properties: Undefined component |
Solutions
*Trace element solution (SL-10) (see Medium [M2073])
Dose: 2 G_PER_L
Cross-reference to Medium M2073
**Se/W solution (see Medium [M2073])
Dose: 2 G_PER_L
Cross-reference to Medium M2073
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.205046Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2993 |
| 2026-01-28T19:48:30.105691Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:50.021189+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:06.478112Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009511 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.483021+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPM" to "ppm" for consistent matching |
| 2026-05-16T09:03:16.341138+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:39.602740+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:29.009622+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-20T19:01:23.970543+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |