Overview
- External ontology
- TOGO:M2996 — PPLO (pleuropneumonia-like organism) medium
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2996 records one liter of PPLO medium with 10 mg/L NAD and growth at 37 C in a 5% CO2 atmosphere.
Composition
flowchart LR
CultureMech_009512["pplo_pleuropneumonia_like_organism_medium"]
ing0_PPLO__pleuropneumonia_like_organism__medium["PPLO (pleuropneumonia-like organism) medium"]
CultureMech_009512 --> ing0_PPLO__pleuropneumonia_like_organism__medium
ing1_NAD["NAD"]
CultureMech_009512 --> ing1_NAD
CHEBI_15846["CHEBI:15846"]
ing1_NAD -.-> CHEBI_15846
ing2_CO2["CO2"]
CultureMech_009512 --> ing2_CO2
CHEBI_16526["CHEBI:16526"]
ing2_CO2 -.-> CHEBI_16526
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | PPLO (pleuropneumonia-like organism) medium | 1000 ML_PER_L | TOGO M2996 lists 1.0 L PPLO medium from Difco. | |
| 2 |
NAD
C21H28N7O14P2 664.438 g/mol
|
10 MG_PER_L |
CHEBI:15846
NAD(+)
|
TOGO M2996 states PPLO medium was supplemented with NAD at 10 ug/mL. |
| 3 |
CO2
CO2 44.009 g/mol
|
5 PERCENT_V_V |
CHEBI:16526
carbon dioxide
|
TOGO M2996 records growth in a 5% CO2 atmosphere. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.445900Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2996 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 |
| 2026-03-13T06:20:06.481816Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009512 |
| 2026-03-17T19:46:25.487466+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPLO (pleuropneumonia-like organism) medium" to "pplo_pleuropneumonia_like_organism_medium" for consistent matching |
| 2026-05-16T09:04:29.394963+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-10T00:00:00-07:00 | repair_togo_m1546_m2996_score20.py | RESOLVED_TOGO_M1546_M2996_SCORE20 | TOGO M2996 records one liter of PPLO medium with 10 mg/L NAD and growth at 37 C in a 5% CO2 atmosphere. |