Overview
- External ontology
- TOGO:M2997 — Acidiphilium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2997 Original source: JCM - JCM_M129-1 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=129 Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009513["acidiphilium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009513 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009513 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_009513 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KCl["KCl"]
CultureMech_009513 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_009513 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_009513 --> ing5_Pancreatic_digest_of_casein
ing6_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_009513 --> ing6_Peptic_digest_of_soybean_meal
ing7_Glucose["Glucose"]
CultureMech_009513 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_Sodium_chloride["Sodium chloride"]
CultureMech_009513 --> ing8_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing8_Sodium_chloride -.-> CHEBI_26710
ing9_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_009513 --> ing9_Dipotassium_phosphate
ing9_Dipotassium_phosphate -.-> CHEBI_131527
ing10_Agar["Agar"]
CultureMech_009513 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_Glucose["Glucose"]
CultureMech_009513 --> ing11_Glucose
ing11_Glucose -.-> CHEBI_17234
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
500.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Collapsed 2 identical duplicates: 500.0, 500.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 7 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 8 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 9 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 10 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 11 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 12 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
Solutions
Solution A
Dose: 500 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 500 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.799694Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M2997 |
| 2026-01-28T19:48:35.933354Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:59.865481+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:31.656952Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:06.486901Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009513 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.493060+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acidiphilium Medium" to "acidiphilium_medium" for consistent matching |
| 2026-05-16T09:03:16.353029+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:39.614061+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:29.021362+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.035468+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:48.337024+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |