2_glucose_yeast_extract_peptone_agar

CultureMech:009517 bacterial COMPLEX SOLID_AGAR
Original name: 2% Glucose-Yeast Extract-Peptone Agar

Overview

External ontology
TOGO:M29 — 2% Glucose-Yeast Extract-Peptone Agar
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M29 Original source: JCM - JCM_M37 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=37

Composition

flowchart LR
    CultureMech_009517["2_glucose_yeast_extract_peptone_agar"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009517 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009517 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009517 --> ing2_Yeast_extract
    ing3_K2HPO4["K2HPO4"]
    CultureMech_009517 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_009517 --> ing4_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_7H2O -.-> CHEBI_75836
    ing5_CaCO3["CaCO3"]
    CultureMech_009517 --> ing5_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing5_CaCO3 -.-> CHEBI_3311
    ing6_Glucose["Glucose"]
    CultureMech_009517 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Agar["Agar"]
    CultureMech_009517 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Peptone["Peptone"]
    CultureMech_009517 --> ing8_Peptone

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
50 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 CaCO3
CO3.Ca 100.086 g/mol
1 G_PER_L CHEBI:3311
calcium carbonate
 Role: Protective agent, Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
8 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
9 Peptone 5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.773613Z togo-import Imported from TOGO Medium Source: JCM, ID: M29
2026-01-28T19:48:10.875829Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:06.523507Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009517
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.532118+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "2% Glucose-Yeast Extract-Peptone Agar" to "2_glucose_yeast_extract_peptone_agar" for consistent matching
2026-06-05T07:40:15.743694+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:12.485917+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:42.534502+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.