Overview
- External ontology
- TOGO:M3016 — Freshwater Thiosulfate-Oxidizing Bacteria Medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.0, 'max': 6.5}
- Notes
- TOGO M3016 records JCM Medium 1347 with Modified Wolfe's solution from JCM Medium 628, Trace minerals from JCM Medium 151, and sterile additions of 1 M MES, 1 M NaHCO3, Trace vitamins from JCM Medium 197, and 1 M Na2S2O3 x 5H2O before pH check and a 79:20:1 N2-CO2-O2 gas phase.
Composition
flowchart LR
CultureMech_009532["freshwater_thiosulfate_oxidizing_bacteria_medium"]
ing0_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009532 --> ing0_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing0_Carbon_dioxide_gas -.-> CHEBI_16526
ing1_Nitrogen_gas["Nitrogen gas"]
CultureMech_009532 --> ing1_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing1_Nitrogen_gas -.-> CHEBI_17997
ing2_Oxygen_gas["Oxygen gas"]
CultureMech_009532 --> ing2_Oxygen_gas
CHEBI_15379["CHEBI:15379"]
ing2_Oxygen_gas -.-> CHEBI_15379
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
JCM Medium 1347 replaces the gas phase with N2-CO2-O2 (79:20:1, v/v/v). |
| 2 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
JCM Medium 1347 replaces the gas phase with N2-CO2-O2 (79:20:1, v/v/v). |
| 3 |
Oxygen gas
O2 31.998 g/mol
|
variable VARIABLE |
CHEBI:15379
dioxygen
|
JCM Medium 1347 replaces the gas phase with N2-CO2-O2 (79:20:1, v/v/v). |
Preparation
- Mix 1 L Modified Wolfe's solution with 10 ml Trace minerals.
- Autoclave the base at 121 degrees C for 15 min.
- Aseptically add sterile stocks per liter: 10 ml 1 M MES solution, 5 ml filter-sterilized 1 M NaHCO3 solution, 10 ml filter-sterilized Trace vitamins, and 10 ml filter-sterilized 1 M Na2S2O3 x 5H2O solution.
- Check the final pH is 6.0-6.5.
- Dispense into culture vessels, replace the gas phase with N2-CO2-O2 (79:20:1, v/v/v), and seal with butyl rubber stoppers.
Solutions
Modified Wolfe's solution
Dose: 1000.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NH4Cl | 1.0 G_PER_L | TOGO M641 / JCM Medium 628 lists 1.0 g/L NH4Cl. |
| MgSO4 x 7H2O | 0.2 G_PER_L | TOGO M641 / JCM Medium 628 lists 0.2 g/L MgSO4 x 7H2O. |
| CaCl2 x 2H2O | 0.1 G_PER_L | TOGO M641 / JCM Medium 628 lists 0.1 g/L CaCl2 x 2H2O. |
| KH2PO4 | 0.05 G_PER_L | TOGO M641 / JCM Medium 628 lists 0.05 g/L KH2PO4. |
| Distilled water | 1.0 L | TOGO M641 / JCM Medium 628 lists 1.0 L Distilled water. |
JCM Medium 1347 lists 1.0 L Modified Wolfe's solution.
Trace minerals
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | 1.5 G_PER_L | TOGO M142 / JCM Medium 151 lists 1.5 g/L Nitrilotriacetic acid. |
| MgSO4 x 7H2O | 3.0 G_PER_L | TOGO M142 / JCM Medium 151 lists 3.0 g/L MgSO4 x 7H2O. |
| MnSO4 x xH2O | 0.5 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.5 g/L MnSO4 x xH2O. |
| NaCl | 1.0 G_PER_L | TOGO M142 / JCM Medium 151 lists 1.0 g/L NaCl. |
| FeSO4 x 7H2O | 0.1 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.1 g/L FeSO4 x 7H2O. |
| CoSO4 x 7H2O | 0.1 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.1 g/L CoSO4 x 7H2O. |
| CaCl2 x 2H2O | 0.1 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.1 g/L CaCl2 x 2H2O. |
| ZnSO4 x 7H2O | 0.1 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.1 g/L ZnSO4 x 7H2O. |
| CuSO4 x 5H2O | 0.01 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.01 g/L CuSO4 x 5H2O. |
| AlK(SO4)2 | 0.01 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.01 g/L AlK(SO4)2. |
| H3BO3 | 0.01 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.01 g/L H3BO3. |
| Na2MoO4 x 2H2O | 0.01 G_PER_L | TOGO M142 / JCM Medium 151 lists 0.01 g/L Na2MoO4 x 2H2O. |
| Distilled water | 1.0 L | TOGO M142 / JCM Medium 151 lists 1.0 L Distilled water. |
JCM Medium 1347 lists 10.0 ml/L Trace minerals.
1 M MES solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MES | 1.0 MOLAR | TOGO M3016 / JCM Medium 1347 lists 1.0 M MES. |
JCM Medium 1347 adds 10.0 ml/L sterile 1 M MES solution.
1 M NaHCO3 solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 1.0 MOLAR | TOGO M3016 / JCM Medium 1347 lists 1.0 M NaHCO3. |
JCM Medium 1347 adds 5.0 ml/L filter-sterilized 1 M NaHCO3 solution.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 2.0 mg/L Biotin. |
| Folic acid | 2.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 2.0 mg/L Folic acid. |
| Pyridoxine HCl | 10.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 10.0 mg/L Pyridoxine HCl. |
| Thiamine HCl | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Thiamine HCl. |
| Riboflavin | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Riboflavin. |
| Nicotinic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Nicotinic acid. |
| Calcium pantothenate | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Calcium pantothenate. |
| Vitamin B12 | 0.1 MG_PER_L | TOGO M190 / JCM Medium 197 lists 0.1 mg/L Vitamin B12. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L p-Aminobenzoic acid. |
| Lipoic acid | 5.0 MG_PER_L | TOGO M190 / JCM Medium 197 lists 5.0 mg/L Lipoic acid. |
| Distilled water | 1.0 L | TOGO M190 / JCM Medium 197 lists 1.0 L Distilled water. |
JCM Medium 1347 adds 10.0 ml/L filter-sterilized Trace vitamins.
1 M Na2S2O3 x 5H2O solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S2O3 x 5H2O | 1.0 MOLAR | TOGO M3016 / JCM Medium 1347 lists 1.0 M Na2S2O3 x 5H2O. |
JCM Medium 1347 adds 10.0 ml/L filter-sterilized 1 M Na2S2O3 x 5H2O solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.687374Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3016 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:05:10.478435+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:20:06.643986Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009532 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T19:46:25.660095+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Freshwater Thiosulfate-Oxidizing Bacteria Medium" to "freshwater_thiosulfate_oxidizing_bacteria_medium" for consistent matching |
| 2026-05-16T09:03:35.220428+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-09-12T00:00:00-07:00 | repair_togo_m3016_score15.py | RESOLVED_TOGO_M3016_SCORE15 | TOGO M3016 records JCM Medium 1347 with Modified Wolfe's solution from JCM Medium 628, Trace minerals from JCM Medium 151, and sterile additions of 1 M MES, 1 M NaHCO3, Trace vitamins from JCM Medium 197, and 1 M Na2S2O3 x 5H2O before pH check and a 79:20:1 N2-CO2-O2 gas phase. |