Overview
- External ontology
- TOGO:M3018 — Modified M9 Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3018 Original source: JCM - JCM_M1355 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1355
Composition
flowchart LR
CultureMech_009533["modified_m9_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009533 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009533 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NH4Cl["NH4Cl"]
CultureMech_009533 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_KCl["KCl"]
CultureMech_009533 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Na2HPO4["Na2HPO4"]
CultureMech_009533 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_NaH2PO4["NaH2PO4"]
CultureMech_009533 --> ing5_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing5_NaH2PO4 -.-> CHEBI_37585
ing6_N2["N2"]
CultureMech_009533 --> ing6_N2
CHEBI_17997["CHEBI:17997"]
ing6_N2 -.-> CHEBI_17997
ing7_Modified_trace_minerals__see_below_["Modified trace minerals (see below)"]
CultureMech_009533 --> ing7_Modified_trace_minerals__see_below_
ing8_Choline_chloride["Choline chloride"]
CultureMech_009533 --> ing8_Choline_chloride
CHEBI_133341["CHEBI:133341"]
ing8_Choline_chloride -.-> CHEBI_133341
ing9_Nicotinamide["Nicotinamide"]
CultureMech_009533 --> ing9_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing9_Nicotinamide -.-> CHEBI_17154
ing10_myo_Inositol["myo-Inositol"]
CultureMech_009533 --> ing10_myo_Inositol
CHEBI_17268["CHEBI:17268"]
ing10_myo_Inositol -.-> CHEBI_17268
ing11_Sodium_ascorbate["Sodium ascorbate"]
CultureMech_009533 --> ing11_Sodium_ascorbate
CHEBI_113451["CHEBI:113451"]
ing11_Sodium_ascorbate -.-> CHEBI_113451
ing12_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009533 --> ing12_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_2H2O -.-> CHEBI_86158
ing13_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_009533 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009533 --> ing14_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing14_MgCl2_6H2O -.-> CHEBI_86345
ing15_H3BO3["H3BO3"]
CultureMech_009533 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_009533 --> ing16_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing16_CoCl2_6H2O -.-> CHEBI_35696
ing17_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009533 --> ing17_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_2H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_009533 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009533 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_MnCl2_2H2O["MnCl2·2H2O"]
CultureMech_009533 --> ing20_MnCl2_2H2O
CHEBI_131395["CHEBI:131395"]
ing20_MnCl2_2H2O -.-> CHEBI_131395
ing21_AlCl3["AlCl3"]
CultureMech_009533 --> ing21_AlCl3
CHEBI_30114["CHEBI:30114"]
ing21_AlCl3 -.-> CHEBI_30114
ing22_FeCl2_4H2O["FeCl2·4H2O"]
CultureMech_009533 --> ing22_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing22_FeCl2_4H2O -.-> CHEBI_86249
ing23_KOH["KOH"]
CultureMech_009533 --> ing23_KOH
CHEBI_32035["CHEBI:32035"]
ing23_KOH -.-> CHEBI_32035
ing24_NaHCO3["NaHCO3"]
CultureMech_009533 --> ing24_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing24_NaHCO3 -.-> CHEBI_32139
ing25_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_009533 --> ing25_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing25_FeCl3_6H2O -.-> CHEBI_86254
ing26_Nitrilotriacetic_acid_trisodium_salt_monohydrate["Nitrilotriacetic acid trisodium salt monohydrate"]
CultureMech_009533 --> ing26_Nitrilotriacetic_acid_trisodium_salt_monohydrate
ing27_NaOH["NaOH"]
CultureMech_009533 --> ing27_NaOH
CHEBI_32145["CHEBI:32145"]
ing27_NaOH -.-> CHEBI_32145
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
936.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 835.0, 1.0, 100.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
21.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 20.0, 1.0] |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.31 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.13 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
4.33 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
2.69 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 8 | Modified trace minerals (see below) | 5 G_PER_L | ||
| 9 |
Choline chloride
C5H14NO.Cl 139.626 g/mol
|
5 G_PER_L |
CHEBI:133341
choline chloride
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 10 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
5 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 11 |
myo-Inositol
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17268
myo-inositol
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 12 |
Sodium ascorbate
C6H7O6.Na 198.106 g/mol
|
5 G_PER_L |
CHEBI:113451
sodium ascorbate
|
Properties: Defined component, Organic compound, Simple component |
| 13 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 21 |
MnCl2·2H2O
Cl2Mn.2H2O 161.874 g/mol
|
0.5 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
AlCl3
AlCl3 133.341 g/mol
|
0.01 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 25 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.64 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
2.7 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 | Nitrilotriacetic acid trisodium salt monohydrate | 2.75 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 28 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
10% Na2CO3 solution
Dose: 40 G_PER_L
Role: Buffer; Properties: Inorganic compound, Defined component, Solution
1 M Sodium acetate solution
Dose: 10 G_PER_L
Role: Buffer; Properties: Organic compound, Defined component, Solution
Modified trace vitamins solution* (see below)
Dose: 10 G_PER_L
Fe NTA solution (see below)
Dose: 100 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:53.688528Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M3018 |
| 2026-01-28T19:48:39.535610Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: KOH; Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:06.486180+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:38.237890Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:06.657262Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009533 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.673665+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified M9 Medium" to "modified_m9_medium" for consistent matching |
| 2026-05-16T09:04:25.622603+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:36.998372+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.692556+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |