Overview
- External ontology
- TOGO:M3037 — Modified synthetic Crenarchaeota medium for strain SCM1
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3037 Original source: NBRC - NBRC_M1378 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1378
Composition
flowchart LR
CultureMech_009549["modified_synthetic_crenarchaeota_medium_for_strain_scm1"]
ing0_Distilled_water["Distilled water"]
CultureMech_009549 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009549 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009549 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009549 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009549 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_KBr["KBr"]
CultureMech_009549 --> ing5_KBr
CHEBI_32030["CHEBI:32030"]
ing5_KBr -.-> CHEBI_32030
ing6_HEPES["HEPES"]
CultureMech_009549 --> ing6_HEPES
CHEBI_46756["CHEBI:46756"]
ing6_HEPES -.-> CHEBI_46756
ing7_NaOH["NaOH"]
CultureMech_009549 --> ing7_NaOH
CHEBI_32145["CHEBI:32145"]
ing7_NaOH -.-> CHEBI_32145
ing8_NaHCO3["NaHCO3"]
CultureMech_009549 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_KH2PO4["KH2PO4"]
CultureMech_009549 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10_FeNaEDTA["FeNaEDTA"]
CultureMech_009549 --> ing10_FeNaEDTA
ing11_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_009549 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_009549 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_009549 --> ing13_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_4H2O -.-> CHEBI_86368
ing14_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_009549 --> ing14_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing14_CoCl2_6H2O -.-> CHEBI_35696
ing15_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_009549 --> ing15_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_6H2O -.-> CHEBI_34887
ing16_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_009549 --> ing16_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_7H2O -.-> CHEBI_32312
ing17_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009549 --> ing17_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_2H2O -.-> CHEBI_86318
ing18_HCl__35__["HCl (35%)"]
CultureMech_009549 --> ing18_HCl__35__
ing19_NH4Cl["NH4Cl"]
CultureMech_009549 --> ing19_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing19_NH4Cl -.-> CHEBI_31206
ing20_Sodium_alpha_ketoglutamate["Sodium alpha-ketoglutamate"]
CultureMech_009549 --> ing20_Sodium_alpha_ketoglutamate
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
305.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 8 duplicates: 1.0, 1.0, 100.0, 1.0, 1.0, 1.0, 100.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
26 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KBr
Br.K 119.002 g/mol
|
0.1 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | HEPES | 238.4 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 8 |
NaOH
HO.Na 39.997 g/mol
|
24 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
8.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 | FeNaEDTA | 578 G_PER_L | Properties: Organic compound, Simple component, Defined component | |
| 12 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
variable VARIABLE |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
variable VARIABLE |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 14 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
variable VARIABLE |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 15 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
variable VARIABLE |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 16 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
variable VARIABLE |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 17 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
variable VARIABLE |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
variable VARIABLE |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 19 | HCl (35%) | variable VARIABLE | Role: Solvating media; Properties: Inorganic compound, Solution, Defined component | |
| 20 |
NH4Cl
Cl.H4N 53.492 g/mol
|
2.14 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | Sodium alpha-ketoglutamate | 0.34 G_PER_L | Properties: Simple component, Defined component |
Solutions
HEPES buffer*
Dose: 10 G_PER_L
1 M NaHCO3 buffer**
Dose: 5 G_PER_L
KH2PO4 solution***
Dose: 5 G_PER_L
FeNaEDTA solution****
Dose: 5 G_PER_L
Trace element solution*****
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
NH4Cl solution******
Dose: 2.5 G_PER_L
Sodium alpha-Ketoglutamate solution*******
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.215783Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3037 |
| 2026-01-28T19:48:25.042833Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Na2MoO4·2H2O; Added default concentration for ingredient: H3BO3; Added default concentration for ingredient: MnCl2·4H2O; Added default concentration for ingredient: CoCl2·6H2O; Added default concentration for ingredient: NiCl2·6H2O; Added default concentration for ingredient: ZnSO4·7H2O; Added default concentration for ingredient: CuCl2·2H2O; Added default concentration for ingredient: HCl (35%) |
| 2026-03-09T08:04:41.425340+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:09:11.435162Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 8 duplicate ingredient(s) |
| 2026-03-13T06:20:06.820644Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009549 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.845074+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified synthetic Crenarchaeota medium for strain SCM1" to "modified_synthetic_crenarchaeota_medium_for_strain_scm1" for consistent matching |
| 2026-05-16T09:04:26.281295+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:37.825959+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:26.827298+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.968924+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |