Overview
- External ontology
- TOGO:M3039 — HGAM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- NBRC Medium 1509 HGAM lists 41.7 g Nissui Modified GAM Broth, 15 g agar if needed, 1.0 L distilled water, and pH 7.3. TOGO M3039 imports the solid agar form from NBRC M1509-1.
Composition
flowchart LR
CultureMech_009551["hgam"]
ing0_Nissui_Modified_GAM_Broth["Nissui Modified GAM Broth"]
CultureMech_009551 --> ing0_Nissui_Modified_GAM_Broth
ing1_Distilled_water["Distilled water"]
CultureMech_009551 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_009551 --> ing2_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing2_Agar__if_needed_ -.-> CHEBI_2509
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Nissui Modified GAM Broth | 41.7 G_PER_L | TOGO M3039 / NBRC Medium 1509 lists 41.7 g/L Nissui Modified GAM Broth; this commercial medium product is retained as an opaque complex component. | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1000.0 ML_PER_L |
CHEBI:15377
water
|
TOGO M3039 / NBRC Medium 1509 lists 1.0 L distilled water. |
| 3 | Agar (if needed) | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M3039 / NBRC Medium 1509 lists 15.0 g/L agar if needed. |
Preparation
- Suspend 41.7 g Nissui Modified GAM Broth and 15.0 g agar, if a solid medium is needed, in 1.0 L distilled water.
- Adjust pH to 7.3.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.222785Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3039 |
| 2026-01-28T19:48:18.309259Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:30.332243+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:06.843958Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009551 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.869686+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HGAM" to "hgam" for consistent matching |
| 2026-05-16T09:03:37.150758+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:08.491895+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m3039_m3040_hgam_score15.py | RESOLVED_TOGO_M3039_HGAM_SCORE15 | Restored the opaque Nissui Modified GAM Broth component from NBRC Medium 1509, corrected the imported water unit to 1.0 L, grounded optional agar, added pH 7.3, and replaced unrelated sparse-signature SOURCE_DUPLICATE children with the true TOGO M3040 anaerobic liquid variant. |