Overview
- External ontology
- TOGO:M3046 — BCYEα-2216E
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3046 Original source: NBRC - NBRC_M1596 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1596
Composition
flowchart LR
CultureMech_009559["bcye_2216e"]
ing0_Distilled_water["Distilled water"]
CultureMech_009559 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_L_Cysteine["L-Cysteine"]
CultureMech_009559 --> ing1_L_Cysteine
CHEBI_17561["CHEBI:17561"]
ing1_L_Cysteine -.-> CHEBI_17561
ing2_Glucose["Glucose"]
CultureMech_009559 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_ACES_Buffer["ACES Buffer"]
CultureMech_009559 --> ing3_ACES_Buffer
ing4_KOH["KOH"]
CultureMech_009559 --> ing4_KOH
CHEBI_32035["CHEBI:32035"]
ing4_KOH -.-> CHEBI_32035
ing5_Ferric_pyrophosphate["Ferric pyrophosphate"]
CultureMech_009559 --> ing5_Ferric_pyrophosphate
CHEBI_132767["CHEBI:132767"]
ing5_Ferric_pyrophosphate -.-> CHEBI_132767
ing6___Ketoglutarate["α-Ketoglutarate"]
CultureMech_009559 --> ing6___Ketoglutarate
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
L-Cysteine
C3H7NO2S 121.161 g/mol
|
1 G_PER_L |
CHEBI:17561
L-cysteine
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 4 | ACES Buffer | 10 G_PER_L | Role: Buffer; Properties: Defined component, Organic compound, Simple component | |
| 5 |
KOH
HO.K 56.105 g/mol
|
2.8 G_PER_L |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
Ferric pyrophosphate
4Fe.3O7P2 745.203 g/mol
|
0.25 G_PER_L |
CHEBI:132767
ferric pyrophosphate
|
Role: Mineral source |
| 7 | α-Ketoglutarate | 1 G_PER_L | Role: Growth factor |
Solutions
Marine Broth 2216**
Dose: 37.4 G_PER_L
Properties: Complex component, Undefined component
Legionella CYE Agar Base*
Dose: 25 G_PER_L
Properties: Complex component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.232547Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3046 |
| 2026-01-28T19:48:12.441334Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:20.510598+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:06.901024Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009559 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.924768+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BCYEα-2216E" to "bcye_2216e" for consistent matching |
| 2026-05-16T09:03:25.563973+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:50.678052+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |