Overview
- External ontology
- TOGO:M304 — Pyrobaculum Arsenaticum Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M304 Original source: JCM - JCM_M309 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=309
Composition
flowchart LR
CultureMech_009563["pyrobaculum_arsenaticum_medium"]
ing0_Resazurin["Resazurin"]
CultureMech_009563 --> ing0_Resazurin
CHEBI_8806["CHEBI:8806"]
ing0_Resazurin -.-> CHEBI_8806
ing1_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009563 --> ing1_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing1_Na2S_9H2O -.-> CHEBI_76209
ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009563 --> ing2_Yeast_extract__BD_Difco_
ing3_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_009563 --> ing3_Bacto_peptone__BD_Difco_
ing4_Na2S2O3["Na2S2O3"]
CultureMech_009563 --> ing4_Na2S2O3
CHEBI_32150["CHEBI:32150"]
ing4_Na2S2O3 -.-> CHEBI_32150
ing5_H2SO4["H2SO4"]
CultureMech_009563 --> ing5_H2SO4
CHEBI_26836["CHEBI:26836"]
ing5_H2SO4 -.-> CHEBI_26836
ing6_N2["N2"]
CultureMech_009563 --> ing6_N2
CHEBI_17997["CHEBI:17997"]
ing6_N2 -.-> CHEBI_17997
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 2 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Simple component, Defined component |
| 3 | Yeast extract (BD-Difco) | 0.2 G_PER_L | Properties: Undefined component | |
| 4 | Bacto peptone (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 5 |
Na2S2O3
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Simple component, Inorganic compound |
| 6 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 7 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Modified Brock's salt base solution (see Medium [M156])
Dose: 1 G_PER_L
Cross-reference to Medium M156
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.818872Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M304 |
| 2026-01-28T19:48:34.726803Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:57.675375+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:06.925119Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009563 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.949173+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pyrobaculum Arsenaticum Medium" to "pyrobaculum_arsenaticum_medium" for consistent matching |
| 2026-05-16T09:03:16.572775+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.842002+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |