mrs_2_fructose_apple_juice

CultureMech:009564 bacterial COMPLEX SOLID_AGAR
Original name: MRS + 2% Fructose + Apple Juice

Overview

External ontology
TOGO:M3050 — MRS + 2% Fructose + Apple Juice
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3050 Original source: NBRC - NBRC_M1600 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1600

Composition

flowchart LR
    CultureMech_009564["mrs_2_fructose_apple_juice"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009564 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_009564 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009564 --> ing2_Yeast_extract
    ing3_K2HPO4["K2HPO4"]
    CultureMech_009564 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Sodium_acetate["Sodium acetate"]
    CultureMech_009564 --> ing4_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate -.-> CHEBI_32954
    ing5_Tween_80["Tween 80"]
    CultureMech_009564 --> ing5_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing5_Tween_80 -.-> CHEBI_53426
    ing6_MnSO4_nH2O["MnSO4·nH2O"]
    CultureMech_009564 --> ing6_MnSO4_nH2O
    CHEBI_86360["CHEBI:86360"]
    ing6_MnSO4_nH2O -.-> CHEBI_86360
    ing7_Diammonium_hydrogen_citrate["Diammonium hydrogen citrate"]
    CultureMech_009564 --> ing7_Diammonium_hydrogen_citrate
    CHEBI_63076["CHEBI:63076"]
    ing7_Diammonium_hydrogen_citrate -.-> CHEBI_63076
    ing8_Fructose["Fructose"]
    CultureMech_009564 --> ing8_Fructose
    CHEBI_28645["CHEBI:28645"]
    ing8_Fructose -.-> CHEBI_28645
    ing9_L_Cysteine["L-Cysteine"]
    CultureMech_009564 --> ing9_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing9_L_Cysteine -.-> CHEBI_17561
    ing10_Glucose["Glucose"]
    CultureMech_009564 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Apple_Juice["Apple Juice"]
    CultureMech_009564 --> ing11_Apple_Juice
    ing12_Agar__if_needed_["Agar (if needed)"]
    CultureMech_009564 --> ing12_Agar__if_needed_
    ing13_Meat_extract["Meat extract"]
    CultureMech_009564 --> ing13_Meat_extract
    ing14_Peptone["Peptone"]
    CultureMech_009564 --> ing14_Peptone

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
3 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
4 K2HPO4
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 Sodium acetate
C2H3O2.Na 82.034 g/mol
5 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Organic compound, Simple component, Defined component
6 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
 Role: Surfactant; Properties: Defined component, Simple component, Organic compound
7 MnSO4·nH2O
Mn.O4S 151.001 g/mol
0.05 G_PER_L CHEBI:86360
manganese(II) sulfate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
8 Diammonium hydrogen citrate
C6H6O7.2H4N 226.185 g/mol
2 G_PER_L CHEBI:63076
diammonium citrate
 Role: Nutrient source; Properties: Defined component, Organic compound, Simple component
9 Fructose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:28645
beta-D-fructofuranose
 Role: Carbon source; Properties: Defined component, Organic compound, Simple component
10 L-Cysteine
C3H7NO2S 121.161 g/mol
1 G_PER_L CHEBI:17561
L-cysteine
 Role: Nitrogen source; Properties: Simple component, Defined component, Organic compound
11 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Defined component, Organic compound, Simple component
12 Apple Juice 200 G_PER_L Role: Nutrient source; Properties: Complex component
13 Agar (if needed) 15 G_PER_L Role: Solidifying component
14 Meat extract 10 G_PER_L Properties: Undefined component
15 Peptone 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:59.238109Z togo-import Imported from TOGO Medium Source: NBRC, ID: M3050
2026-01-28T19:48:24.328801Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:06.932533Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009564
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:25.956443+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS + 2% Fructose + Apple Juice" to "mrs_2_fructose_apple_juice" for consistent matching
2026-06-05T07:41:38.408048+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:27.244888+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:09.168750+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.