Overview
- External ontology
- TOGO:M3054 — LB + 1% Tween 80
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3054 Original source: NBRC - NBRC_M1608 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1608
Composition
flowchart LR
CultureMech_009568["lb_1_tween_80"]
ing0_Distilled_water["Distilled water"]
CultureMech_009568 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009568 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009568 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Tween_80["Tween 80"]
CultureMech_009568 --> ing3_Tween_80
CHEBI_53426["CHEBI:53426"]
ing3_Tween_80 -.-> CHEBI_53426
ing4_Agar__if_needed_["Agar (if needed)"]
CultureMech_009568 --> ing4_Agar__if_needed_
ing5_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_009568 --> ing5_Bacto_Tryptone__Difco_
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
10 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Simple component, Organic compound, Defined component |
| 5 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 6 | Bacto Tryptone (Difco) | 10 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.246012Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3054 |
| 2026-01-28T19:48:41.513607Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:06.967196Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009568 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.990837+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB + 1% Tween 80" to "lb_1_tween_80" for consistent matching |
| 2026-06-05T07:41:10.787020+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |