Overview
- External ontology
- TOGO:M3057 — Azospira Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3057 Original source: NBRC - NBRC_M1620 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1620
Composition
flowchart LR
CultureMech_009571["azospira_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009571 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009571 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009571 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KH2PO4["KH2PO4"]
CultureMech_009571 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_009571 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KCl["KCl"]
CultureMech_009571 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaHCO3["NaHCO3"]
CultureMech_009571 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_CH3COONa["CH3COONa"]
CultureMech_009571 --> ing7_CH3COONa
ing8_NaMoO4_2H2O["NaMoO4･2H2O"]
CultureMech_009571 --> ing8_NaMoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing8_NaMoO4_2H2O -.-> CHEBI_75213
ing9_H3BO4["H3BO4"]
CultureMech_009571 --> ing9_H3BO4
ing10_FeSO4_7H2O["FeSO4･7H2O"]
CultureMech_009571 --> ing10_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_7H2O -.-> CHEBI_75836
ing11_MnCl2_4H2O["MnCl2･4H2O"]
CultureMech_009571 --> ing11_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_4H2O -.-> CHEBI_86368
ing12_ZnSO4_7H2O["ZnSO4･7H2O"]
CultureMech_009571 --> ing12_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_7H2O -.-> CHEBI_32312
ing13_CuSO4_7H2O["CuSO4･7H2O"]
CultureMech_009571 --> ing13_CuSO4_7H2O
ing14_FeCl3_6H2O["FeCl3･6H2O"]
CultureMech_009571 --> ing14_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing14_FeCl3_6H2O -.-> CHEBI_86254
ing15_Na2SeO3["Na2SeO3"]
CultureMech_009571 --> ing15_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing15_Na2SeO3 -.-> CHEBI_48843
ing16_citric_acid["citric acid"]
CultureMech_009571 --> ing16_citric_acid
CHEBI_30769["CHEBI:30769"]
ing16_citric_acid -.-> CHEBI_30769
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
8.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
6.6 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
100 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
115 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
13.4 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
188 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 | CH3COONa | 200 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 9 |
NaMoO4･2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.5 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 | H3BO4 | 0.1 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 11 |
FeSO4･7H2O
Fe.7H2O.O4S 278.013 g/mol
|
10 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MnCl2･4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
ZnSO4･7H2O
7H2O.O4S.Zn 287.558 g/mol
|
2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | CuSO4･7H2O | 4 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 15 |
FeCl3･6H2O
Cl3Fe.6H2O 270.294 g/mol
|
9.38 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.3 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
citric acid
C6H8O7 192.123 g/mol
|
10 G_PER_L |
CHEBI:30769
citric acid
|
Properties: Defined component, Organic compound, Simple component |
Solutions
*trace elements solution
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.248820Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M3057 |
| 2026-01-28T19:48:34.484854Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Distilled water |
| 2026-03-09T08:04:57.257589+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:28.835498Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:06.982584Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009571 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.006479+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Azospira Medium" to "azospira_medium" for consistent matching |
| 2026-05-16T09:03:25.218774+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:50.146821+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:37.047803+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:37:34.564492+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:53.668098+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |