msgg_medium_for_matrix_production_of_nbrc_xxxxxx

CultureMech:009572 bacterial COMPLEX SOLID_AGAR
Original name: MSgg medium for matrix production of NBRC XXXXXX

Overview

External ontology
TOGO:M3058 — MSgg medium for matrix production of NBRC XXXXXX
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3058 Original source: NBRC - NBRC_M1625 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1625

Composition

flowchart LR
    CultureMech_009572["msgg_medium_for_matrix_production_of_nbrc_xxxxxx"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009572 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_009572 --> ing1_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_2H2O -.-> CHEBI_86158
    ing2_KH2PO4["KH2PO4"]
    CultureMech_009572 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_009572 --> ing3_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_6H2O -.-> CHEBI_86345
    ing4_MnCl3_4H2O["MnCl3·4H2O"]
    CultureMech_009572 --> ing4_MnCl3_4H2O
    ing5_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_009572 --> ing5_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing5_Thiamine_hydrochloride -.-> CHEBI_49105
    ing6_ZnCl2["ZnCl2"]
    CultureMech_009572 --> ing6_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing6_ZnCl2 -.-> CHEBI_49976
    ing7_FeCl3_6H2O["FeCl3·6H2O"]
    CultureMech_009572 --> ing7_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing7_FeCl3_6H2O -.-> CHEBI_86254
    ing8_Glycerol["Glycerol"]
    CultureMech_009572 --> ing8_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing8_Glycerol -.-> CHEBI_17754
    ing9_L_glutamic_acid_monosodium_salt_monohydrate["L-glutamic acid monosodium salt monohydrate"]
    CultureMech_009572 --> ing9_L_glutamic_acid_monosodium_salt_monohydrate
    ing10_MOPS["MOPS"]
    CultureMech_009572 --> ing10_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing10_MOPS -.-> CHEBI_39074
    ing11_Tryptophan["Tryptophan"]
    CultureMech_009572 --> ing11_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing11_Tryptophan -.-> CHEBI_27897
    ing12_Phenylalanine["Phenylalanine"]
    CultureMech_009572 --> ing12_Phenylalanine
    CHEBI_17295["CHEBI:17295"]
    ing12_Phenylalanine -.-> CHEBI_17295
    ing13_Threonine["Threonine"]
    CultureMech_009572 --> ing13_Threonine
    CHEBI_26986["CHEBI:26986"]
    ing13_Threonine -.-> CHEBI_26986
    ing14_Agar__if_needed_["Agar (if needed)"]
    CultureMech_009572 --> ing14_Agar__if_needed_

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.7 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 KH2PO4
H2O4P.K 136.084 g/mol
5 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
2 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 MnCl3·4H2O 0.05 MILLIMOLAR Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
2 MICROMOLAR CHEBI:49105
thiamine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
7 ZnCl2
2Cl.Zn 136.296 g/mol
1 MICROMOLAR CHEBI:49976
zinc dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
0.05 MILLIMOLAR CHEBI:86254
iron trichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 Glycerol
C3H8O3 92.094 g/mol
5 G_PER_L CHEBI:17754
glycerol
 Role: Carbon source; Properties: Organic compound, Defined component, Simple component
10 L-glutamic acid monosodium salt monohydrate 5 G_PER_L Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
11 MOPS 100 MILLIMOLAR CHEBI:39074
MOPS
 Role: Buffer; Properties: Organic compound, Defined component, Simple component
12 Tryptophan
C11H12N2O2 204.229 g/mol
50 G_PER_L CHEBI:27897
tryptophan
 Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
13 Phenylalanine
C9H11NO2 165.192 g/mol
50 G_PER_L CHEBI:17295
L-phenylalanine
 Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
14 Threonine
C4H9NO3 119.119 g/mol
50 G_PER_L CHEBI:26986
threonine
 Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
15 Agar (if needed) 20 G_PER_L Role: Solidifying component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:59.251161Z togo-import Imported from TOGO Medium Source: NBRC, ID: M3058
2026-01-28T19:48:15.688450Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:06.991579Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009572
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.016082+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MSgg medium for matrix production of NBRC XXXXXX" to "msgg_medium_for_matrix_production_of_nbrc_xxxxxx" for consistent matching
2026-06-05T07:41:38.785936+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.262044+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:09.253487+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-20T19:02:59.272689+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).