complete_medium

CultureMech:009573 bacterial COMPLEX SOLID_AGAR
Original name: Complete Medium

Overview

External ontology
TOGO:M3059 — Complete Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3059 Original source: NBRC - NBRC_M1627 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1627

Composition

flowchart LR
    CultureMech_009573["complete_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009573 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_009573 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009573 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_009573 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_009573 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Sodium_citrate["Sodium citrate"]
    CultureMech_009573 --> ing5_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing5_Sodium_citrate -.-> CHEBI_53258
    ing6_4_98__FeSO4["4.98% FeSO4"]
    CultureMech_009573 --> ing6_4_98__FeSO4
    ing7_Casein["Casein"]
    CultureMech_009573 --> ing7_Casein
    ing8_Agar__if_needed_["Agar (if needed)"]
    CultureMech_009573 --> ing8_Agar__if_needed_

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
10 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Yeast extract 10 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
150 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 Sodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
Role: Buffer; Properties: Organic compound, Simple component, Defined component
7 4.98% FeSO4 1 G_PER_L Role: Mineral source; Properties: Solution, Defined component, Inorganic compound
8 Casein 7.5 G_PER_L Role: Nitrogen source; Properties: Undefined component
9 Agar (if needed) 15 G_PER_L Role: Solidifying component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable).
2026-01-27T06:35:59.253137Z togo-import Imported from TOGO Medium Source: NBRC, ID: M3059
2026-01-28T19:48:12.118106Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:06.998374Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009573
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.023181+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Complete Medium" to "complete_medium" for consistent matching
2026-06-05T07:40:54.753687+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:39.986883+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.