nutrient_agar

CultureMech:009588 bacterial COMPLEX SOLID_AGAR
Original name: Nutrient Agar

Overview

External ontology
TOGO:M3116 — Nutrient Agar
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M3116 / DSMZ Medium 1 defines Nutrient Agar using DSMZ Medium 1. DSMZ Medium 1 lists, per liter, 5 g Peptone, 3 g Meat extract, 15 g Agar if necessary, and 1000 ml Distilled water. Adjust pH to 7.0.

Composition

flowchart LR
    CultureMech_009588["nutrient_agar"]
    ing0_Peptone["Peptone"]
    CultureMech_009588 --> ing0_Peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_009588 --> ing1_Meat_extract
    ing2_Agar__if_necessary["Agar, if necessary"]
    CultureMech_009588 --> ing2_Agar__if_necessary
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar__if_necessary -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_009588 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Peptone 5.0 G_PER_L MICRO:0000178 (source link unavailable)
Peptone
DSMZ Medium 1 lists 5 g/L Peptone.
2 Meat extract 3.0 G_PER_L DSMZ Medium 1 lists 3 g/L Meat extract; this generic complex extract is retained without an ontology grounding.
3 Agar, if necessary 15.0 G_PER_L CHEBI:2509
agar
DSMZ Medium 1 lists 15 g/L Agar, if necessary.
4 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
DSMZ Medium 1 lists 1000 ml Distilled water.

Preparation

  1. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.450899Z togo-import Imported from TOGO Medium Source: TOGO, ID: M3116
2026-01-28T19:48:07.812787Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:07.096250Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009588
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.122499+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nutrient Agar" to "nutrient_agar" for consistent matching
2026-06-05T07:40:39.863959+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m3116_score15.py RESOLVED_TOGO_M3116_SCORE15 TOGO M3116 / DSMZ Medium 1 defines Nutrient Agar using DSMZ Medium 1. DSMZ Medium 1 lists, per liter, 5 g Peptone, 3 g Meat extract, 15 g Agar if necessary, and 1000 ml Distilled water. Adjust pH to 7.0. Corrected the imported water unit artifact, grounded the disclosed simple and protein-hydrolysate components, kept Meat extract intentionally unmapped, and linked the record to DSMZ Medium 1.