Overview
- External ontology
- TOGO:M3127 — MMJS medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3127
Composition
flowchart LR
CultureMech_009597["mmjs_medium"]
ing0_Elemental_sulfer["Elemental sulfer"]
CultureMech_009597 --> ing0_Elemental_sulfer
ing1_Resazurin["Resazurin"]
CultureMech_009597 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Na2S_9H2O["Na2S.9H2O"]
CultureMech_009597 --> ing2_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing2_Na2S_9H2O -.-> CHEBI_76209
ing3_NaHCO3["NaHCO3"]
CultureMech_009597 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Sodium_selenite["Sodium selenite"]
CultureMech_009597 --> ing4_Sodium_selenite
CHEBI_48843["CHEBI:48843"]
ing4_Sodium_selenite -.-> CHEBI_48843
ing5_MJ_synthetic_seawater["MJ synthetic seawater"]
CultureMech_009597 --> ing5_MJ_synthetic_seawater
ing6_Cysteine_hydrochloride["Cysteine hydrochloride"]
CultureMech_009597 --> ing6_Cysteine_hydrochloride
CHEBI_91248["CHEBI:91248"]
ing6_Cysteine_hydrochloride -.-> CHEBI_91248
ing7_NaOH["NaOH"]
CultureMech_009597 --> ing7_NaOH
CHEBI_32145["CHEBI:32145"]
ing7_NaOH -.-> CHEBI_32145
ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009597 --> ing8_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing8_Carbon_dioxide_gas -.-> CHEBI_16526
ing9_Hydrogen_gas["Hydrogen gas"]
CultureMech_009597 --> ing9_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing9_Hydrogen_gas -.-> CHEBI_18276
ing10_Distilled_water["Distilled water"]
CultureMech_009597 --> ing10_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing10_Distilled_water -.-> CHEBI_15377
ing11_Biotin["Biotin"]
CultureMech_009597 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009597 --> ing12_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
ing13_Thiamine_hydrochloride["Thiamine hydrochloride"]
CultureMech_009597 --> ing13_Thiamine_hydrochloride
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_hydrochloride -.-> CHEBI_49105
ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009597 --> ing14_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing15_Folic_acid["Folic acid"]
CultureMech_009597 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Vitamine_B12["Vitamine B12"]
CultureMech_009597 --> ing16_Vitamine_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamine_B12 -.-> CHEBI_176843
ing17_Riboflavin["Riboflavin"]
CultureMech_009597 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_009597 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_Lipoic_acid["Lipoic acid"]
CultureMech_009597 --> ing19_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing19_Lipoic_acid -.-> CHEBI_16494
ing20_DL_calcium_pantothenate["DL-calcium pantothenate"]
CultureMech_009597 --> ing20_DL_calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_DL_calcium_pantothenate -.-> CHEBI_31345
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Elemental sulfer | 3 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component | |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 3 |
Na2S.9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
20 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Sodium selenite
2Na.O3Se 172.937 g/mol
|
50 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 | MJ synthetic seawater | 1 G_PER_L | Role: Mineral source, Solvating media; Properties: Defined component, Complex component | |
| 7 |
Cysteine hydrochloride
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 8 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 10 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
| 11 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 | Vitamine B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 21 |
DL-calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Vitamin solution
Dose: 1 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.476080Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3127 |
| 2026-01-28T19:48:07.002804Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:11.295852+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:07.223860Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009597 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.251669+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJS medium" to "mmjs_medium" for consistent matching |
| 2026-05-16T09:03:16.716571+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.988555+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |