Overview
- External ontology
- TOGO:M3141 — Chloroflexus Medium (modified)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3141 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87.pdf
Composition
flowchart LR
CultureMech_009609["chloroflexus_medium_modified"]
ing0_Distilled_water["Distilled water"]
CultureMech_009609 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009609 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009609 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009609 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009609 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_NaNO3["NaNO3"]
CultureMech_009609 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_KNO3["KNO3"]
CultureMech_009609 --> ing6_KNO3
CHEBI_63043["CHEBI:63043"]
ing6_KNO3 -.-> CHEBI_63043
ing7_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_009609 --> ing7_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing7_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing8_Glycyl_glycine["Glycyl-glycine"]
CultureMech_009609 --> ing8_Glycyl_glycine
CHEBI_17201["CHEBI:17201"]
ing8_Glycyl_glycine -.-> CHEBI_17201
ing9_N2_gas["N2 gas"]
CultureMech_009609 --> ing9_N2_gas
ing10_Fe_III__citrate["Fe(III) citrate"]
CultureMech_009609 --> ing10_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing10_Fe_III__citrate -.-> CHEBI_144421
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009609 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_2_M_H2SO4["2 M H2SO4"]
CultureMech_009609 --> ing12_2_M_H2SO4
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009609 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_009609 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009609 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009609 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009609 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009609 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009609 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2250.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 1050.0, 100.0, 100.0, 1000.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.5 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.1 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Glycyl-glycine
C4H8N2O3 132.119 g/mol
|
1 G_PER_L |
CHEBI:17201
glycylglycine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 10 | N2 gas | variable VARIABLE | Properties: ガス | |
| 11 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.1 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 13 | 2 M H2SO4 | variable VARIABLE | Role: Mineral source; Properties: Defined component, Inorganic compound, Solution | |
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Neutralized sulfide solution
Dose: 0.03 PERCENT_W_V
Fe(III) citrate solution
Dose: 5 G_PER_L
Trace element solution SL-6
Dose: 1 G_PER_L
See mediadive_25_Trace_element_solution_SL-6.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.516183Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3141 |
| 2026-01-28T19:48:07.161532Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: 2 M H2SO4; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:11.559278+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:38.298613Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:07.428788Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009609 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.456116+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chloroflexus Medium (modified)" to "chloroflexus_medium_modified" for consistent matching |
| 2026-05-16T09:03:16.854078+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.126377+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:29.480646+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.209145+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |