Overview
- External ontology
- TOGO:M3145 — 1/2 SME medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3145
Composition
flowchart LR
CultureMech_009611["1_2_sme_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009611 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_009611 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009611 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2.2H2O"]
CultureMech_009611 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009611 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_MgCl2_6H2O["MgCl2.6H2O"]
CultureMech_009611 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_H3BO3["H3BO3"]
CultureMech_009611 --> ing6_H3BO3
CHEBI_33118["CHEBI:33118"]
ing6_H3BO3 -.-> CHEBI_33118
ing7_KCl["KCl"]
CultureMech_009611 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_009611 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9__NH4_2SO4["(NH4)2SO4"]
CultureMech_009611 --> ing9__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing9__NH4_2SO4 -.-> CHEBI_62946
ing10_Elemental_sulfur["Elemental sulfur"]
CultureMech_009611 --> ing10_Elemental_sulfur
CHEBI_33403["CHEBI:33403"]
ing10_Elemental_sulfur -.-> CHEBI_33403
ing11_NaBr["NaBr"]
CultureMech_009611 --> ing11_NaBr
CHEBI_63004["CHEBI:63004"]
ing11_NaBr -.-> CHEBI_63004
ing12_SrCl2_6H2O["SrCl2.6H2O"]
CultureMech_009611 --> ing12_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing12_SrCl2_6H2O -.-> CHEBI_36385
ing13_KI["KI"]
CultureMech_009611 --> ing13_KI
CHEBI_8346["CHEBI:8346"]
ing13_KI -.-> CHEBI_8346
ing14_Na2S["Na2S"]
CultureMech_009611 --> ing14_Na2S
CHEBI_76208["CHEBI:76208"]
ing14_Na2S -.-> CHEBI_76208
ing15_sulfuric_acid["sulfuric acid"]
CultureMech_009611 --> ing15_sulfuric_acid
CHEBI_26836["CHEBI:26836"]
ing15_sulfuric_acid -.-> CHEBI_26836
ing16_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009611 --> ing16_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing16_Carbon_dioxide_gas -.-> CHEBI_16526
ing17_Hydrogen_gas["Hydrogen gas"]
CultureMech_009611 --> ing17_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing17_Hydrogen_gas -.-> CHEBI_18276
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
13.85 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2.2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.38 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
MgCl2.6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
H3BO3
H3BO3 61.833 g/mol
|
0.015 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.25 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Elemental sulfur | 5 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
SrCl2.6H2O
Cl2Sr.6H2O 266.616 g/mol
|
7.5 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
KI
I.K 166.002 g/mol
|
25 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2S
2Na.S 78.047 g/mol
|
0.5 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
sulfuric acid
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 17 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 18 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.522393Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3145 |
| 2026-01-28T19:48:13.484836Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: sulfuric acid; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-13T06:20:07.452417Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009611 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.479363+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/2 SME medium" to "1_2_sme_medium" for consistent matching |
| 2026-05-16T09:03:16.880142+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:40.153002+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.220637+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |