Overview
- External ontology
- TOGO:M3149 — DSM 135 medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3149
Composition
flowchart LR
CultureMech_009613["dsm_135_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_009613 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_009613 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Resazurin["Resazurin"]
CultureMech_009613 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Distilled_water["Distilled water"]
CultureMech_009613 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_Yeast_extract["Yeast extract"]
CultureMech_009613 --> ing4_Yeast_extract
ing5_Ammonium_chloride["Ammonium chloride"]
CultureMech_009613 --> ing5_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing5_Ammonium_chloride -.-> CHEBI_31206
ing6_Sodium_bicarbonate["Sodium bicarbonate"]
CultureMech_009613 --> ing6_Sodium_bicarbonate
CHEBI_32139["CHEBI:32139"]
ing6_Sodium_bicarbonate -.-> CHEBI_32139
ing7_Magnesium_sulfate_heptahydrate["Magnesium sulfate heptahydrate"]
CultureMech_009613 --> ing7_Magnesium_sulfate_heptahydrate
CHEBI_31795["CHEBI:31795"]
ing7_Magnesium_sulfate_heptahydrate -.-> CHEBI_31795
ing8_Cysteine_hydrochloride["Cysteine-hydrochloride"]
CultureMech_009613 --> ing8_Cysteine_hydrochloride
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009613 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Hydrogen_gas["Hydrogen gas"]
CultureMech_009613 --> ing10_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing10_Hydrogen_gas -.-> CHEBI_18276
ing11_Biotin["Biotin"]
CultureMech_009613 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Lipoic_acid["Lipoic acid"]
CultureMech_009613 --> ing12_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing12_Lipoic_acid -.-> CHEBI_16494
ing13_Thiamine_HCl["Thiamine-HCl"]
CultureMech_009613 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_009613 --> ing14_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_HCl -.-> CHEBI_30961
ing15_Folic_acid["Folic acid"]
CultureMech_009613 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_009613 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_Riboflavin["Riboflavin"]
CultureMech_009613 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_009613 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_Pantothenate["Pantothenate"]
CultureMech_009613 --> ing19_Pantothenate
CHEBI_16454["CHEBI:16454"]
ing19_Pantothenate -.-> CHEBI_16454
ing20_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_009613 --> ing20_MgSO4_7H2O
ing20_MgSO4_7H2O -.-> CHEBI_31795
ing21_NaCl["NaCl"]
CultureMech_009613 --> ing21_NaCl
CHEBI_26710["CHEBI:26710"]
ing21_NaCl -.-> CHEBI_26710
ing22_Na2MO4_2H2O["Na2MO4.2H2O"]
CultureMech_009613 --> ing22_Na2MO4_2H2O
ing23_H3BO3["H3BO3"]
CultureMech_009613 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_FeSO4_7H2O["FeSO4.7H2O"]
CultureMech_009613 --> ing24_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing24_FeSO4_7H2O -.-> CHEBI_75836
ing25_NiCl2_6H2O["NiCl2.6H2O"]
CultureMech_009613 --> ing25_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing25_NiCl2_6H2O -.-> CHEBI_34887
ing26_ZnSO4_7H2O["ZnSO4.7H2O"]
CultureMech_009613 --> ing26_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing26_ZnSO4_7H2O -.-> CHEBI_32312
ing27_CuSO4_5H2O["CuSO4.5H2O"]
CultureMech_009613 --> ing27_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing27_CuSO4_5H2O -.-> CHEBI_31440
ing28_Na2SeO3_5H2O["Na2SeO3.5H2O"]
CultureMech_009613 --> ing28_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing28_Na2SeO3_5H2O -.-> CHEBI_131361
ing29_CoSO4_7H2O["CoSO4.7H2O"]
CultureMech_009613 --> ing29_CoSO4_7H2O
CHEBI_53470["CHEBI:53470"]
ing29_CoSO4_7H2O -.-> CHEBI_53470
CultureMech_009613 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
4.6 MILLIMOLAR |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5.4 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
4 MICROMOLAR |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 5 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Sodium bicarbonate
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Magnesium sulfate heptahydrate
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution |
| 9 | Cysteine-hydrochloride | 0.3 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
4 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
10 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
10 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
20 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
4 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 | Vitamin B12 | 0.2 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
10 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
10 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Pantothenate
C9H16NO5 218.229 g/mol
|
10 G_PER_L |
CHEBI:16454
pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 | Na2MO4.2H2O | 10 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
10 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
NiCl2.6H2O
Cl2Ni 129.599 g/mol
|
30 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
ZnSO4.7H2O
7H2O.O4S.Zn 287.558 g/mol
|
180 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
CuSO4.5H2O
Cu.5H2O.O4S 249.684 g/mol
|
10 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
Na2SeO3.5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
CoSO4.7H2O
Co.O4S 154.996 g/mol
|
180 G_PER_L |
CHEBI:53470
cobalt(2+) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 32 |
Na2WO4.2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
MnSO4.H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Vitamin solution
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Trace element solution
Dose: 20 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.528669Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3149 |
| 2026-01-28T19:48:27.555283Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:45.620673+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:16.101218Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:07.488077Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009613 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.513859+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DSM 135 medium" to "dsm_135_medium" for consistent matching |
| 2026-05-16T09:03:31.057981+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:58.997183+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:37.935024+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:56.505407+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:03.982026+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |