Overview
- External ontology
- TOGO:M3155 — PETC-MES medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3155
Composition
flowchart LR
CultureMech_009617["petc_mes_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009617 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009617 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009617 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_009617 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_009617 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Fe_SO4__7H2O["Fe(SO4)·7H2O"]
CultureMech_009617 --> ing5_Fe_SO4__7H2O
ing6_KCl["KCl"]
CultureMech_009617 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Sodium_acetate["Sodium acetate"]
CultureMech_009617 --> ing7_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing7_Sodium_acetate -.-> CHEBI_32954
ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009617 --> ing8_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
ing9_CaCl2["CaCl2"]
CultureMech_009617 --> ing9_CaCl2
CHEBI_3312["CHEBI:3312"]
ing9_CaCl2 -.-> CHEBI_3312
ing10_Fructose["Fructose"]
CultureMech_009617 --> ing10_Fructose
CHEBI_28645["CHEBI:28645"]
ing10_Fructose -.-> CHEBI_28645
ing11_MES["MES"]
CultureMech_009617 --> ing11_MES
CHEBI_39010["CHEBI:39010"]
ing11_MES -.-> CHEBI_39010
ing12_2_g_L_Resazurin["2 g/L Resazurin"]
CultureMech_009617 --> ing12_2_g_L_Resazurin
ing13_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_009617 --> ing13_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing13_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing14_NaMoO4_2H2O["NaMoO4·2H2O"]
CultureMech_009617 --> ing14_NaMoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing14_NaMoO4_2H2O -.-> CHEBI_75213
ing15_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_009617 --> ing15_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2_6H2O -.-> CHEBI_35696
ing16_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_009617 --> ing16_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_6H2O -.-> CHEBI_34887
ing17_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_009617 --> ing17_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_7H2O -.-> CHEBI_32312
ing18_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009617 --> ing18_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_2H2O -.-> CHEBI_86318
ing19_Na2SeO3_5H2O["Na2SeO3·5H2O"]
CultureMech_009617 --> ing19_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing19_Na2SeO3_5H2O -.-> CHEBI_131361
ing20_Na2WO4_2H2O["Na2WO4·2H2O"]
CultureMech_009617 --> ing20_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_2H2O -.-> CHEBI_63939
ing21_MnSO4_H2O["MnSO4·H2O"]
CultureMech_009617 --> ing21_MnSO4_H2O
CHEBI_86364["CHEBI:86364"]
ing21_MnSO4_H2O -.-> CHEBI_86364
ing22_Biotin["Biotin"]
CultureMech_009617 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_4_Aminobenzoic_acid["4-Aminobenzoic acid"]
CultureMech_009617 --> ing23_4_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_4_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Thiamine_HCl["Thiamine-HCl"]
CultureMech_009617 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009617 --> ing25_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_pantothenate -.-> CHEBI_31345
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009617 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Folic_acid["Folic acid"]
CultureMech_009617 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_009617 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_Riboflavin["Riboflavin"]
CultureMech_009617 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_009617 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 1.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 | Fe(SO4)·7H2O | 0.8500000000000001 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.05, 0.8] | |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.25 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 9 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
2.05 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 0.05, 2.0] |
| 10 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.02 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Fructose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 12 | MES | 20 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 13 | 2 g/L Resazurin | 0.5 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 14 |
Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.4 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 15 |
NaMoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.02 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2SeO3·5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.02 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
MnSO4·H2O
H2O.Mn.O4S 169.016 g/mol
|
1 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
4-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace metal solution
Dose: 10 G_PER_L
See mediadive_6140_Trace_metal_solution.yaml for composition
Wolfe’s vitamin solution
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.541398Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3155 |
| 2026-01-28T19:48:16.704418Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:27.602626+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:56.345423Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:07.553319Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009617 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.576417+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PETC-MES medium" to "petc_mes_medium" for consistent matching |
| 2026-05-16T09:04:28.778605+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:41.415549+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:29.297004+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.057556+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:55.049545+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |