Overview
- External ontology
- TOGO:M3167 — LC medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3167
Composition
flowchart LR
CultureMech_009624["lc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009624 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009624 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_009624 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_009624 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_009624 --> ing4_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing4_Na2MoO4_2H2O -.-> CHEBI_75213
ing5_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009624 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_H3BO3["H3BO3"]
CultureMech_009624 --> ing6_H3BO3
CHEBI_33118["CHEBI:33118"]
ing6_H3BO3 -.-> CHEBI_33118
ing7_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_009624 --> ing7_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_7H2O -.-> CHEBI_75836
ing8_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_009624 --> ing8_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_4H2O -.-> CHEBI_86368
ing9_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_009624 --> ing9_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing9_CoCl2_6H2O -.-> CHEBI_35696
ing10_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_009624 --> ing10_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_6H2O -.-> CHEBI_34887
ing11_PABA["PABA"]
CultureMech_009624 --> ing11_PABA
CHEBI_30753["CHEBI:30753"]
ing11_PABA -.-> CHEBI_30753
ing12_B12["B12"]
CultureMech_009624 --> ing12_B12
ing13_ZnCl2["ZnCl2"]
CultureMech_009624 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_009624 --> ing14_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_5H2O -.-> CHEBI_31440
ing15_Na2SO4["Na2SO4"]
CultureMech_009624 --> ing15_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing15_Na2SO4 -.-> CHEBI_32149
ing16_FeCl2_4H2O["FeCl2·4H2O"]
CultureMech_009624 --> ing16_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing16_FeCl2_4H2O -.-> CHEBI_86249
ing17_Pyridoxamine_dihydrochloride["Pyridoxamine dihydrochloride"]
CultureMech_009624 --> ing17_Pyridoxamine_dihydrochloride
CHEBI_131532["CHEBI:131532"]
ing17_Pyridoxamine_dihydrochloride -.-> CHEBI_131532
ing18_D_Biotin["D-Biotin"]
CultureMech_009624 --> ing18_D_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_D_Biotin -.-> CHEBI_15956
ing19_Urea["Urea"]
CultureMech_009624 --> ing19_Urea
CHEBI_16199["CHEBI:16199"]
ing19_Urea -.-> CHEBI_16199
ing20_L_cysteine_HCl_H2O["L-cysteine HCl.H2O"]
CultureMech_009624 --> ing20_L_cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing20_L_cysteine_HCl_H2O -.-> CHEBI_91248
ing21_4_N_KOH["4 N KOH"]
CultureMech_009624 --> ing21_4_N_KOH
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.6 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
H3BO3
H3BO3 61.833 g/mol
|
0.6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0035 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
6 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.6 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.6 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
PABA
C7H7NO2 137.138 g/mol
|
4 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 | B12 | 2 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
2.5 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.6 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.1 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.025 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Pyridoxamine dihydrochloride
C8H12N2O2.2HCl 241.118 g/mol
|
20 G_PER_L |
CHEBI:131532
pyridoxamine dihydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
D-Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Urea
CH4N2O 60.056 g/mol
|
0.5 G_PER_L |
CHEBI:16199
urea
|
Properties: Defined component, Organic compound, Simple component |
| 21 |
L-cysteine HCl.H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 22 | 4 N KOH | variable VARIABLE | Role: Mineral source; Properties: Defined component, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.565394Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3167 |
| 2026-01-28T19:48:36.208916Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 4 N KOH |
| 2026-03-13T06:20:07.671921Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009624 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.686270+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LC medium" to "lc_medium" for consistent matching |
| 2026-05-16T09:03:16.928144+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:40.200823+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.239224+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |