Overview
- External ontology
- TOGO:M3171 — Modified MJ Nutrient Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3171
Composition
flowchart LR
CultureMech_009626["modified_mj_nutrient_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009626 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_Extract["Yeast Extract"]
CultureMech_009626 --> ing1_Yeast_Extract
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009626 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_K2HPO4["K2HPO4"]
CultureMech_009626 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KH2PO4["KH2PO4"]
CultureMech_009626 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Resazurin["Resazurin"]
CultureMech_009626 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009626 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_Biotin["Biotin"]
CultureMech_009626 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_p_Aminobenzoic_Acid["p-Aminobenzoic Acid"]
CultureMech_009626 --> ing8_p_Aminobenzoic_Acid
CHEBI_30753["CHEBI:30753"]
ing8_p_Aminobenzoic_Acid -.-> CHEBI_30753
ing9_Sodium_Citrate["Sodium Citrate"]
CultureMech_009626 --> ing9_Sodium_Citrate
CHEBI_53258["CHEBI:53258"]
ing9_Sodium_Citrate -.-> CHEBI_53258
ing10_MOPS["MOPS"]
CultureMech_009626 --> ing10_MOPS
CHEBI_39074["CHEBI:39074"]
ing10_MOPS -.-> CHEBI_39074
ing11_FeSO4_6H2O["FeSO4·6H2O"]
CultureMech_009626 --> ing11_FeSO4_6H2O
CHEBI_75832["CHEBI:75832"]
ing11_FeSO4_6H2O -.-> CHEBI_75832
ing12_Pyridoxamine_HCl["Pyridoxamine-HCl"]
CultureMech_009626 --> ing12_Pyridoxamine_HCl
CHEBI_131531["CHEBI:131531"]
ing12_Pyridoxamine_HCl -.-> CHEBI_131531
ing13_Cyanocobalamin["Cyanocobalamin"]
CultureMech_009626 --> ing13_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing13_Cyanocobalamin -.-> CHEBI_17439
ing14_Urea["Urea"]
CultureMech_009626 --> ing14_Urea
CHEBI_16199["CHEBI:16199"]
ing14_Urea -.-> CHEBI_16199
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast Extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
150 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 7 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 9 |
p-Aminobenzoic Acid
C7H7NO2 137.138 g/mol
|
40 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
| 10 |
Sodium Citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 11 | MOPS | 10 G_PER_L |
CHEBI:39074
MOPS
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 12 |
FeSO4·6H2O
Fe.O4S 151.908 g/mol
|
1.25 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 |
Pyridoxamine-HCl
C8H12N2O2.HCl 204.657 g/mol
|
200 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
| 14 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
20 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin |
| 15 |
Urea
CH4N2O 60.056 g/mol
|
2.1 G_PER_L |
CHEBI:16199
urea
|
Properties: Defined component, Organic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.568002Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3171 |
| 2026-01-28T19:48:32.454188Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:07.686722Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009626 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.700920+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified MJ Nutrient Medium" to "modified_mj_nutrient_medium" for consistent matching |
| 2026-06-05T07:41:37.321301+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.804162+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |