minimal_nitrification_medium_nm (CultureMech:009629)

Overview

External ontology

TOGO:M3179 — Minimal nitrification medium (NM)

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: https://togomedium.org/medium/M3179

Composition

flowchart LR
    CultureMech_009629["minimal_nitrification_medium_nm"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009629 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_KH2PO4["KH2PO4"]
    CultureMech_009629 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009629 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_CaCl2["CaCl2"]
    CultureMech_009629 --> ing3_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing3_CaCl2 -.-> CHEBI_3312
    ing4_MgSO4["MgSO4"]
    CultureMech_009629 --> ing4_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing4_MgSO4 -.-> CHEBI_32599
    ing5_CuSO4["CuSO4"]
    CultureMech_009629 --> ing5_CuSO4
    CHEBI_23414["CHEBI:23414"]
    ing5_CuSO4 -.-> CHEBI_23414
    ing6_Na2CO3["Na2CO3"]
    CultureMech_009629 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_FeSO4["FeSO4"]
    CultureMech_009629 --> ing7_FeSO4
    CHEBI_75832["CHEBI:75832"]
    ing7_FeSO4 -.-> CHEBI_75832
    ing8_NaH2PO4["NaH2PO4"]
    CultureMech_009629 --> ing8_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing8_NaH2PO4 -.-> CHEBI_37585
    ing9_EDTA["EDTA"]
    CultureMech_009629 --> ing9_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing9_EDTA -.-> CHEBI_4735

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2	KH2PO4 H2O4P.K 136.084 g/mol	5.85 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
3	(NH4)2SO4 2H4N.O4S 132.141 g/mol	3.3 G_PER_L	CHEBI:62946 ammonium sulfate	Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
4	CaCl2 Ca.2Cl 110.984 g/mol	22 G_PER_L	CHEBI:3312 calcium dichloride	Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
5	MgSO4 Mg.O4S 120.368 g/mol	90 G_PER_L	CHEBI:32599 magnesium sulfate	Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
6	CuSO4 Cu.O4S 159.609 g/mol	80 G_PER_L	CHEBI:23414 copper(II) sulfate	Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7	Na2CO3 CO3.2Na 105.988 g/mol	400 G_PER_L	CHEBI:29377 sodium carbonate	Role: Buffer; Properties: Defined component, Inorganic compound, Solution
8	FeSO4 Fe.O4S 151.908 g/mol	1.5 G_PER_L	CHEBI:75832 iron(2+) sulfate (anhydrous)	Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9	NaH2PO4 H2O4P.Na 119.976 g/mol	0.48 G_PER_L	CHEBI:37585 sodium dihydrogenphosphate	Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
10	EDTA C10H16N2O8 292.244 g/mol	5 G_PER_L	CHEBI:4735 ethylenediaminetetraacetic acid	Properties: Defined component, Organic compound, Simple component

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.576949Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M3179
2026-01-28T19:48:06.986778Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:07.724937Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009629
2026-03-17T06:29:39.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.738665+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Minimal nitrification medium (NM)" to "minimal_nitrification_medium_nm" for consistent matching
2026-06-05T07:41:34.785943+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.078618+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.