Overview
- External ontology
- TOGO:M3181 — Anaerobic butyrate Rhodospirillaceae medium (RM)
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M3181
Composition
flowchart LR
CultureMech_009631["anaerobic_butyrate_rhodospirillaceae_medium_rm"]
ing0_Distilled_water["Distilled water"]
CultureMech_009631 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009631 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009631 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_009631 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009631 --> ing4_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_7H2O -.-> CHEBI_31795
ing5_NaHCO3["NaHCO3"]
CultureMech_009631 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_009631 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_Fe_Citrate["Fe-Citrate"]
CultureMech_009631 --> ing7_Fe_Citrate
ing8_Butyric_acid["Butyric acid"]
CultureMech_009631 --> ing8_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing8_Butyric_acid -.-> CHEBI_30772
ing9_10_M_NaOH["10-M NaOH"]
CultureMech_009631 --> ing9_10_M_NaOH
ing10_Trace_metals["Trace metals"]
CultureMech_009631 --> ing10_Trace_metals
ing11_Trace_vitamins["Trace vitamins"]
CultureMech_009631 --> ing11_Trace_vitamins
ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009631 --> ing12_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing12_Carbon_dioxide_gas -.-> CHEBI_16526
ing13_Nitrogen_gas["Nitrogen gas"]
CultureMech_009631 --> ing13_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing13_Nitrogen_gas -.-> CHEBI_17997
ing14_H3BO3["H3BO3"]
CultureMech_009631 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_009631 --> ing15_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_4H2O -.-> CHEBI_86368
ing16_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_009631 --> ing16_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing16_CoCl2_6H2O -.-> CHEBI_35696
ing17_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_009631 --> ing17_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_6H2O -.-> CHEBI_34887
ing18_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009631 --> ing18_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_2H2O -.-> CHEBI_86318
ing19_ZnCl2["ZnCl2"]
CultureMech_009631 --> ing19_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing19_ZnCl2 -.-> CHEBI_49976
ing20_Concentrated_HCl["Concentrated HCl"]
CultureMech_009631 --> ing20_Concentrated_HCl
ing21_Biotin["Biotin"]
CultureMech_009631 --> ing21_Biotin
CHEBI_15956["CHEBI:15956"]
ing21_Biotin -.-> CHEBI_15956
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009631 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Thiamine["Thiamine"]
CultureMech_009631 --> ing23_Thiamine
CHEBI_18385["CHEBI:18385"]
ing23_Thiamine -.-> CHEBI_18385
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.64 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Fe-Citrate | 0.01 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 9 |
Butyric acid
C4H8O2 88.106 g/mol
|
1 G_PER_L |
CHEBI:30772
butyric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 10 | 10-M NaOH | 1.12 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 11 | Trace metals | 1 G_PER_L | ||
| 12 | Trace vitamins | 1 G_PER_L | ||
| 13 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 14 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
60 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
200 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
20 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
20 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | Concentrated HCl | 0.25 G_PER_L | Role: Solvating media | |
| 22 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
1 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Thiamine
C12H17N4OS 265.362 g/mol
|
1 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.578414Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3181 |
| 2026-01-28T19:48:25.425956Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-13T03:09:12.113516Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:07.745873Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009631 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.757165+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Anaerobic butyrate Rhodospirillaceae medium (RM)" to "anaerobic_butyrate_rhodospirillaceae_medium_rm" for consistent matching |
| 2026-05-16T09:03:24.284700+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:48.845085+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:36.189740+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:53.150387+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:56.298622+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |