Overview
- External ontology
- TOGO:M3202 — Defined minimal medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3202
Composition
flowchart LR
CultureMech_009645["defined_minimal_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009645 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_009645 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_009645 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaNO3["NaNO3"]
CultureMech_009645 --> ing3_NaNO3
CHEBI_63005["CHEBI:63005"]
ing3_NaNO3 -.-> CHEBI_63005
ing4_Na2HPO4["Na2HPO4"]
CultureMech_009645 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_Sodium_Succinate["Sodium Succinate"]
CultureMech_009645 --> ing5_Sodium_Succinate
CHEBI_63675["CHEBI:63675"]
ing5_Sodium_Succinate -.-> CHEBI_63675
ing6_MgSO4["MgSO4"]
CultureMech_009645 --> ing6_MgSO4
CHEBI_32599["CHEBI:32599"]
ing6_MgSO4 -.-> CHEBI_32599
ing7_NaNO2["NaNO2"]
CultureMech_009645 --> ing7_NaNO2
CHEBI_78870["CHEBI:78870"]
ing7_NaNO2 -.-> CHEBI_78870
ing8_N2["N2"]
CultureMech_009645 --> ing8_N2
CHEBI_17997["CHEBI:17997"]
ing8_N2 -.-> CHEBI_17997
ing9_CuSO4["CuSO4"]
CultureMech_009645 --> ing9_CuSO4
CHEBI_23414["CHEBI:23414"]
ing9_CuSO4 -.-> CHEBI_23414
ing10_CoCl2["CoCl2"]
CultureMech_009645 --> ing10_CoCl2
CHEBI_35696["CHEBI:35696"]
ing10_CoCl2 -.-> CHEBI_35696
ing11_FeSO4["FeSO4"]
CultureMech_009645 --> ing11_FeSO4
CHEBI_75832["CHEBI:75832"]
ing11_FeSO4 -.-> CHEBI_75832
ing12_MnCl2["MnCl2"]
CultureMech_009645 --> ing12_MnCl2
CHEBI_63041["CHEBI:63041"]
ing12_MnCl2 -.-> CHEBI_63041
ing13_ZnSO4["ZnSO4"]
CultureMech_009645 --> ing13_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing13_ZnSO4 -.-> CHEBI_35176
ing14__NH4_6Mo7O24["(NH4)6Mo7O24"]
CultureMech_009645 --> ing14__NH4_6Mo7O24
CHEBI_91249["CHEBI:91249"]
ing14__NH4_6Mo7O24 -.-> CHEBI_91249
ing15_EDTA["EDTA"]
CultureMech_009645 --> ing15_EDTA
CHEBI_4735["CHEBI:4735"]
ing15_EDTA -.-> CHEBI_4735
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
variable VARIABLE |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
variable VARIABLE |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
variable VARIABLE |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
variable VARIABLE |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Sodium Succinate
C4H4O4.2Na 162.052 g/mol
|
variable VARIABLE |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgSO4
Mg.O4S 120.368 g/mol
|
variable VARIABLE |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaNO2
NO2.Na 68.995 g/mol
|
variable VARIABLE |
CHEBI:78870
sodium nitrite
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 10 |
CuSO4
Cu.O4S 159.609 g/mol
|
variable VARIABLE |
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CoCl2
2Cl.Co 129.839 g/mol
|
variable VARIABLE |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
FeSO4
Fe.O4S 151.908 g/mol
|
variable VARIABLE |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution |
| 13 |
MnCl2
Cl2Mn 125.844 g/mol
|
variable VARIABLE |
CHEBI:63041
manganese(II) chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnSO4
O4S.Zn 161.453 g/mol
|
variable VARIABLE |
CHEBI:35176
zinc sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
(NH4)6Mo7O24
2H4N.MoO4 196.014 g/mol
|
variable VARIABLE |
CHEBI:91249
ammonium molybdate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
EDTA
C10H16N2O8 292.244 g/mol
|
variable VARIABLE |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Vishniac and Santer trace elements solution
Dose: variable VARIABLE
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.593958Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3202 |
| 2026-01-28T19:48:10.365544Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KH2PO4; Added default concentration for ingredient: NH4Cl; Added default concentration for ingredient: NaNO3; Added default concentration for ingredient: Na2HPO4; Added default concentration for ingredient: Sodium Succinate; Added default concentration for ingredient: MgSO4; Added default concentration for ingredient: NaNO2; Added default concentration for ingredient: N2; Added default concentration for ingredient: Vishniac and Santer trace elements solution; Added default concentration for ingredient: CuSO4; Added default concentration for ingredient: CoCl2; Added default concentration for ingredient: FeSO4; Added default concentration for ingredient: MnCl2; Added default concentration for ingredient: ZnSO4; Added default concentration for ingredient: (NH4)6Mo7O24; Added default concentration for ingredient: EDTA |
| 2026-02-06T05:10:16.224505Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:16.920423+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:44.382864Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:07.864525Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009645 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.871746+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Defined minimal medium" to "defined_minimal_medium" for consistent matching |
| 2026-05-16T09:03:17.047817+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:40.320587+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.287749+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:48.926817+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |