luria_broth_lb

CultureMech:009651 bacterial COMPLEX LIQUID
Original name: Luria Broth (LB)

Overview

External ontology
TOGO:M3210 — Luria Broth (LB)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M3210

Composition

flowchart LR
    CultureMech_009651["luria_broth_lb"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_009651 --> ing0_Yeast_extract
    ing1_NaCl["NaCl"]
    CultureMech_009651 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_Deionized_water["Deionized water"]
    CultureMech_009651 --> ing2_Deionized_water
    ing3_Triptone["Triptone"]
    CultureMech_009651 --> ing3_Triptone
    ing4_HCl["HCl"]
    CultureMech_009651 --> ing4_HCl
    CHEBI_17883["CHEBI:17883"]
    ing4_HCl -.-> CHEBI_17883
    ing5_NaOH["NaOH"]
    CultureMech_009651 --> ing5_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing5_NaOH -.-> CHEBI_32145

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
2 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 Deionized water 1 G_PER_L Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
4 Triptone 10 G_PER_L Properties: Undefined component
5 HCl
HCl 36.461 g/mol
variable VARIABLE CHEBI:17883
hydrogen chloride
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution
6 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.599366Z togo-import Imported from TOGO Medium Source: TOGO, ID: M3210
2026-01-28T19:48:34.021008Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: HCl; Added default concentration for ingredient: NaOH
2026-03-13T06:20:07.894877Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009651
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:26.901503+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Luria Broth (LB)" to "luria_broth_lb" for consistent matching
2026-05-16T09:03:39.291127+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:11.514139+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.