Overview
- External ontology
- TOGO:M3213 — Saline basal medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3213
Composition
flowchart LR
CultureMech_009654["saline_basal_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009654 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Magnesium_sulfate_heptahydrate["Magnesium sulfate heptahydrate"]
CultureMech_009654 --> ing1_Magnesium_sulfate_heptahydrate
CHEBI_31795["CHEBI:31795"]
ing1_Magnesium_sulfate_heptahydrate -.-> CHEBI_31795
ing2_Ammonium_sulfate["Ammonium sulfate"]
CultureMech_009654 --> ing2_Ammonium_sulfate
CHEBI_62946["CHEBI:62946"]
ing2_Ammonium_sulfate -.-> CHEBI_62946
ing3_Fructose["Fructose"]
CultureMech_009654 --> ing3_Fructose
CHEBI_28645["CHEBI:28645"]
ing3_Fructose -.-> CHEBI_28645
ing4_Sodium_monobasic_phosphate["Sodium monobasic phosphate"]
CultureMech_009654 --> ing4_Sodium_monobasic_phosphate
ing5_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
CultureMech_009654 --> ing5_Potassium_dihydrogen_phosphate
CHEBI_63036["CHEBI:63036"]
ing5_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
ing6_1_N_HCl["1 N HCl"]
CultureMech_009654 --> ing6_1_N_HCl
ing7_2_N_NaOH["2 N NaOH"]
CultureMech_009654 --> ing7_2_N_NaOH
ing8_0_1N_HCl["0.1N HCl"]
CultureMech_009654 --> ing8_0_1N_HCl
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009654 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009654 --> ing10_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing11_H3BO3["H3BO3"]
CultureMech_009654 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009654 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009654 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009654 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009654 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_FeSO4["FeSO4"]
CultureMech_009654 --> ing16_FeSO4
CHEBI_75832["CHEBI:75832"]
ing16_FeSO4 -.-> CHEBI_75832
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
Magnesium sulfate heptahydrate
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Ammonium sulfate
2H4N.O4S 132.141 g/mol
|
1.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Fructose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 | Sodium monobasic phosphate | 4.35 G_PER_L | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component | |
| 6 |
Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
|
4.35 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | 1 N HCl | variable VARIABLE | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 8 | 2 N NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 9 | 0.1N HCl | 1 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeSO4
Fe.O4S 151.908 g/mol
|
2 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Microelement solution
Dose: 2 G_PER_L
See mediadive_4239_Microelement_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.603064Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3213 |
| 2026-01-28T19:48:27.163821Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1 N HCl; Added default concentration for ingredient: 2 N NaOH |
| 2026-03-09T08:04:45.039936+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:07.915053Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009654 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:26.920620+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Saline basal medium" to "saline_basal_medium" for consistent matching |
| 2026-05-16T09:04:31.175330+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:45.058682+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.238898+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |