Overview
- External ontology
- TOGO:M322 — Renibacterium KDM-2 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M322 Original source: JCM - JCM_M327 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=327
Composition
flowchart LR
CultureMech_009669["renibacterium_kdm_2_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009669 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Fetal_bovine_serum["Fetal bovine serum"]
CultureMech_009669 --> ing1_Fetal_bovine_serum
ing2_Agar["Agar"]
CultureMech_009669 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009669 --> ing3_Yeast_extract__BD_Difco_
ing4_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_009669 --> ing4_Bacto_peptone__BD_Difco_
ing5_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009669 --> ing5_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing6_NaOH["NaOH"]
CultureMech_009669 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
800 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Fetal bovine serum | 200 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 4 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 5 | Bacto peptone (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 6 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 7 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.851849Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M322 |
| 2026-01-28T19:48:18.343399Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-13T06:20:08.005974Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009669 |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.010399+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Renibacterium KDM-2 Medium" to "renibacterium_kdm_2_medium" for consistent matching |
| 2026-05-16T09:03:17.072687+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:40.346797+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |