Overview
- External ontology
- TOGO:M3231 — Minimal media
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3231
Composition
flowchart LR
CultureMech_009671["minimal_media"]
ing0_Distilled_water["Distilled water"]
CultureMech_009671 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009671 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_009671 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_009671 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_009671 --> ing4_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing4_CuSO4_5H2O -.-> CHEBI_31440
ing5_KCl["KCl"]
CultureMech_009671 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_MnSO4_H2O["MnSO4·H2O"]
CultureMech_009671 --> ing6_MnSO4_H2O
CHEBI_86364["CHEBI:86364"]
ing6_MnSO4_H2O -.-> CHEBI_86364
ing7_FeCl3["FeCl3"]
CultureMech_009671 --> ing7_FeCl3
CHEBI_30808["CHEBI:30808"]
ing7_FeCl3 -.-> CHEBI_30808
ing8_Nitrogen["Nitrogen"]
CultureMech_009671 --> ing8_Nitrogen
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen -.-> CHEBI_17997
ing9_NH4NO3["NH4NO3"]
CultureMech_009671 --> ing9_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing9_NH4NO3 -.-> CHEBI_63038
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
MnSO4·H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.03 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Nitrogen
N2 28.014 g/mol
|
0.1 G_PER_L |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 10 |
NH4NO3
H4N.NO3 80.043 g/mol
|
variable VARIABLE |
CHEBI:63038
ammonium nitrate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.624162Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3231 |
| 2026-01-28T19:48:17.132669Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NH4NO3 |
| 2026-03-13T06:20:08.015020Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009671 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.019433+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Minimal media" to "minimal_media" for consistent matching |
| 2026-05-16T09:03:17.079648+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:40.353790+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:29.681194+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.300735+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |