Overview
- External ontology
- TOGO:M3246 — 9 K medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M3246
Composition
flowchart LR
CultureMech_009679["9_k_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009679 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_009679 --> ing1_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing1_FeSO4_7H2O -.-> CHEBI_75836
ing2_KCl["KCl"]
CultureMech_009679 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_009679 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Ca_NO3_2["Ca(NO3)2"]
CultureMech_009679 --> ing4_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing4_Ca_NO3_2 -.-> CHEBI_64205
ing5_0_1_M_H2SO4["0.1 M H2SO4"]
CultureMech_009679 --> ing5_0_1_M_H2SO4
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
44.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
0.001 G_PER_L |
CHEBI:64205
calcium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 | 0.1 M H2SO4 | variable VARIABLE | Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.632508Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M3246 |
| 2026-01-28T19:48:23.810491Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 0.1 M H2SO4 |
| 2026-03-13T06:20:08.056329Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009679 |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:27.061476+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "9 K medium" to "9_k_medium" for consistent matching |
| 2026-05-16T09:03:17.092106+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:40.370893+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.058994+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:50.306982+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |